J Korean Med Sci.  2018 May;33(21):e102. 10.3346/jkms.2018.33.e102.

Serial Changes of Cytokines in Children with Cerebral Palsy Who Received Intravenous Granulocyte-colony Stimulating Factor Followed by Autologous Mobilized Peripheral Blood Mononuclear Cells

• Koh H
• Rah WJ
• Kim YJ
• Moon JH
• Kim MJ
• Lee YH

• KMID: 2428948
• DOI: http://doi.org/10.3346/jkms.2018.33.e102

Abstract

BACKGROUND
This study was performed to assess serial cytokine changes and their clinical impact in children with cerebral palsy (CP) who received granulocyte-colony stimulating factor (G-CSF) followed by infusion of autologous mobilized peripheral blood mononuclear cells (mPBMCs).
METHODS
Peripheral blood (PB) samples were collected from 16 CP children at enrollment, and 1 month and 7 months after G-CSF infusion as well as at the end of the study. Cytokine levels were measured by enzyme-linked immunosorbent assays with plasma samples.
RESULTS
There were no significant differences in cytokine levels between the mPBMC and placebo groups over 6 months. However, when clinical responders and non-responders were compared, interleukin (IL)-6 (P = 0.050) as well as G-CSF (P = 0.010) were higher in the responders than the non-responders at 1 month, while brain-derived neurotrophic factor (BDNF) (P = 0.030) and insulin-like growth factor (IGF)-1 (P = 0.001) were lower. In addition, BDNF was higher at baseline in the responders than the non-responders (P = 0.030).
CONCLUSION
The changes of G-CSF itself, as well as G-CSF-induced cytokines such as IL-6, may be associated with the clinical improvement of neurologic functions. The G-CSF-induced changes of IL-6, BDNF and IGF-1, and BDNF levels before treatment, could be used as prognostic factors in G-CSF trials in CP children.

Keyword

Cytokines; Granulocyte-colony Stimulating Factor (G-CSF); Peripheral Blood Mononuclear Cells; Cerebral Palsy

MeSH Terms

Brain-Derived Neurotrophic Factor
Cerebral Palsy*
Child*
Cytokines*
Enzyme-Linked Immunosorbent Assay
Granulocyte Colony-Stimulating Factor
Humans
Insulin-Like Growth Factor I
Interleukin-6
Interleukins
Plasma
Brain-Derived Neurotrophic Factor
Cytokines
Granulocyte Colony-Stimulating Factor
Insulin-Like Growth Factor I
Interleukin-6
Interleukins