J Vet Sci.  2018 Nov;19(6):855-857. 10.4142/jvs.2018.19.6.855.

First detection and genetic characterization of porcine parvovirus 7 from Korean domestic pig farms

• Ouh IO
• Park S
• Lee JY
• Song JY
• Cho IS
• Kim HR
• Park CK

• KMID: 2427036
• DOI: http://doi.org/10.4142/jvs.2018.19.6.855

Abstract

Porcine parvovirus 7 (PPV7) was first detected in Korean pig farms in 2017. The detection rate of PPV7 DNA was 24.0% (30/125) in aborted pig fetuses and 74.9% (262/350) in finishing pigs, suggesting that PPV7 has circulated among Korean domestic pig farms. Phylogenetic analysis based on capsid protein amino acid sequences demonstrated that the nine isolated Korean strains (PPV-KA1-3 and PPV-KF1-6) were closely related to the previously reported USA and Chinese PPV7 strains. In addition, the Korean strains exhibit genetic diversity with both insertion and deletion mutations. This study contributes to the understanding of the molecular epidemiology of PPV7 in Korea.

Keyword

Korea; aborted fetus; detection; pig farm; porcine parvovirus 7

MeSH Terms

Aborted Fetus
Agriculture*
Amino Acid Sequence
Asian Continental Ancestry Group
Capsid Proteins
DNA
Fetus
Genetic Variation
Humans
Korea
Molecular Epidemiology
Parvovirus, Porcine*
Sequence Deletion
Sus scrofa*
Swine
Capsid Proteins
DNA

Figure

• 

  Fig. 1 Phylogenetic tree constructed based on the capsid protein (VP) sequences. Phylogenetic tree of VP sequences was derived from 39 Parvovirinae genomes. The tree was inferred from amino acid sequences of the VP by applying the maximum-likelihood method using the LG + F + I model with 1,000 bootstrap resampling iterations. The Korean porcine parvovirus 7 (PPV7) strains identified in this study are represented by black circles.

Reference

1. Le SQ, Gascuel O. An improved general amino acid replacement matrix. Mol Biol Evol. 2008; 25:1307–1320.

2. Lee JY, Kim EJ, Cho IS, Lee KK, Shin YK. Complete genome sequences of porcine parvovirus 2 isolated from swine in the Republic of Korea. Genome Announc. 2017; 5:e01738-16.

3. Oh WT, Kim RY, Nguyen VG, Chung HC, Park BK. Perspectives on the evolution of porcine parvovirus. Viruses. 2017; 9:pii: E196.

4. Palinski RM, Mitra N, Hause BM. Discovery of a novel Parvovirinae virus, porcine parvovirus 7, by metagenomic sequencing of porcine rectal swabs. Virus Genes. 2016; 52:564–567.

5. Streck AF, Canal CW, Truyen U. Molecular epidemiology and evolution of porcine parvoviruses. Infect Genet Evol. 2015; 36:300–306.

6. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013; 30:2725–2729.

7. Xiao CT, Giménez-Lirola LG, Jiang YH, Halbur PG, Opriessnig T. Characterization of a novel porcine parvovirus tentatively designated PPV5. PLoS One. 2013; 8:e65312.

8. Xing X, Zhou H, Tong L, Chen Y, Sun Y, Wang H, Zhang G. First identification of porcine parvovirus 7 in China. Arch Virol. 2018; 163:209–213.