J Cancer Prev.  2018 Sep;23(3):141-146. 10.15430/JCP.2018.23.3.141.

Proteases are Modulated by Fascin in Oral Cancer Invasion

• Lee MK
• Park JH
• Gi SH
• Hwang YS

• KMID: 2425317
• DOI: http://doi.org/10.15430/JCP.2018.23.3.141

Abstract

BACKGROUND
Cancer invasion is a critical factor for survival and prognosis of patients with cancer. Identifying and targeting factors that influence cancer invasion are an important strategy to overcome cancer. In this study, we investigated the role of fascin known to be associated with cancer invasion.
METHODS
Fascin depletion was performed with lentiviral short hairpin RNA against fascin mRNA and stable cell line (Fascin(dep)) was established. Matrigel-Transwell invasion and three-dimensional (3D) culture system were used to observe fascin depletion effects. In order to observe the changes of protease secretion by fascin depleted cancer cells, protease antibody array was performed.
RESULTS
Fascin was highly expressed in invasive cancer cells. Fascin-depleted cells showed decreased cancer invasion in Matrigel-Transwell invasion and 3D culture system. In addition, inhibition of proteases secreation and decrease of intracellular proteases mRNA expression were observed in fascin deplete cells.
CONCLUSIONS
These results indicates that fascin is closely involved in proteases activity and cancer invasion. Therefore, fascin is a strategically important factor for controlling cancer invasion.

Keyword

Metalloproteases; Fascin; Head and neck neoplasms; Gene silencing; Tumor microenvironment

MeSH Terms

Cell Line
Gene Silencing
Head and Neck Neoplasms
Humans
Metalloproteases
Mouth Neoplasms*
Peptide Hydrolases*
Prognosis
RNA, Messenger
RNA, Small Interfering
Tumor Microenvironment
Metalloproteases
Peptide Hydrolases
RNA, Messenger
RNA, Small Interfering