Korean J Nutr.  2009 Dec;42(8):740-749.

Effect of Dietary Intake of Ultra-fine or Nano-Scale Pulverized Cornstarch on the Growing Performance and Gut Function in Rats

• Lee HS
• Ju D
• Kim B
• Kim SH
• Han MR
• Kim MH
• Chang MJ

Abstract

The objectives of this study was to determine whether a new physically modified cornstarch by ultra-fine- or nanoscale pulverizer to reduce particle size offers better bioactive function than native cornstarch in weanling Sprague-Dawley rats. Male weaning Sprague-Dawley rats were fed diets containing native cornstarch (NAC), ultra fine pulverized cornstarch (UFC) or nano-scale pulverized cornstarch (NSC) for 4 weeks. In vitro rate of starch hydrolysis, growth performance, organ weight, intestine length intestinal proliferation and the fermentation by Bifidobacterium of rat cecum were evaluated. The diet with reduced particle size (UFC or NSC) significantly increased body weight gain and organ weight. Feed efficiency was increased in NSC fed rats and was not affected in UFC fed rats. Intestinal proliferation was decreased in NSC group. Reduction of particle size also increased cecal short chain fatty acid concentration and the growth and acidifying activity of Bifidobacterium. It is concluded that a reduction of particle size of starch granules by physically modification may increase growing performance and gut function.

Keyword

cornstarch; ultra-fine pulverization; nano-scale pulverization; growing performance; gut function

MeSH Terms

Animals
Bifidobacterium
Body Weight
Cecum
Diet
Fermentation
Humans
Hydrolysis
Intestines
Male
Organ Size
Particle Size
Rats
Rats, Sprague-Dawley
Starch
Weaning
Starch

Figure

• 

  Fig. 1 In vitro digestibility of native cornstarch, ultra-fine pulverized and nano-scale pulverized cornstarch. NAC: native cornstarch; UFC: ultra-fine pulverized starch, NSC: nano-scale pulverized cornstarch. Data are means and bars indicate SD. Values with different superscripts are significantly different as assessed by Duncan's multiple range test (p < 0.05).

• 

  Fig. 2 Intestinal morphology. A: Duodenal mucosa of a NAC fed rat. B: Duodenal mucosa of a UFC fed rat. C: Duodenal mucosa of a NSC fed rat (decrease in stained cell number), Original magnification, ×100.

Reference

1. Eliasson AC, Gudmundsson M. Eliasson A-C, editor. Starch: physicochemical and functional aspects. Carbohydrates in food. 2006. New York: Marcer Dekker;431–503.

2. CFR, Code of Federal Regulations. Food starch modified. Title21 Chapter 1, Part 172 Sec172.892. Food additives permitted for direct addition to food for human consumption. 2006. Washington, DC: US GPO.

3. Jacobs H, Delcour JA. Hydrothermal modifications of granular starch, with retention of granular structure. A review. J Agric Food Chem. 1998. 46:2895–2905.

4. Holm J, Björck I, Asp NG, Sjöberg LB, Lundquist I. Starch availability in vitro and in vivo after flaking steam-cooking and popping of wheat. J Cereal Sci. 1985. 3:193–206.

5. Stephen AM, Haddad AC, Phillips SF. Passage of carbohydrate into the colon. Direct measurements in human. Gastroenterology. 1983. 85:589–559.

6. O'Riordan K, Muljadi N, Conway P. Characterization of factors affecting attachement of Bifidobacterium species to amylomaize starch granules. J Appl Microbiol. 2001. 90:749–754.

7. Champ M, Deloty-Larval J. Effects of processing on chemical characteristics and nutritive value of cereals. Anim Prod. 1991. 52:564–565.

8. Oberdörster G, Oerdrster E, Oberdrster J. Nanotoxicology: An emerging discipline evolving from studies of ultrafine particles. Environ Health Perspect. 2005. 113:823–839.

9. Sanguansri P, Augustin MA. Nanoscale material development a food industry perspective. Trends Food Sci Technol. 2006. 17:547–556.

10. Park JH, Kim MH, Chang MJ. The effect of dietary ultra finely pulverized rice starch on growth performance and develo0pment of small intestine. Korean J Food Culture. 2007. 22:645–651.

11. Liu H, Corke H, Ramsden L. Functional properties and enzymatic digestibility of cationic and cross-linked cationic ae, wx, and normal maize starch. J Agric Food Chem. 1999. 47(7):2523–2528.

12. Schutte B, Reynders MM, Bosman FT, Glijham GH. Effect of tissue fixation on anti-bromodeoxyuridine immunohistochemisty. J Histochem Cytochen. 1987. 35(11):1343–1345.

13. Chen HL, Lu YH, Lin J, Ko LY. Effects of fructooligosaccharide on bowel function and indicators of nutritional status in constipated elderly men. Nutr Res. 2000. 20(12):1725–1733.

14. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin-Phenol reagent. J Biol Chem. 1951. 193(1):265–275.

15. Thacker PA. Effect of micronization on the performance of growing/finishing pigs fed diets based on hulled and hulless barley. Anim Feed Sci Technol. 1999. 79:29–41.

16. Fernades TH, Hutton K, Smith WC. A note on the use of micronized barley for growing pigs. Anm Prod. 1975. 20:307–310.

17. Huang SX, Sauer WC, Pickard M, Li S, Hardin RT. Effect of micronization on energy, starch and amino acid digestibility in hulless barley for young pigs. Can J Anim Sci. 1998. 78:81–87.

18. Healy BJ, Hancock JD, Kennedy GA, Bramel-Cox PJ, Behnke KC, Hines RH. Optimum particle size of corn and hard and soft sorghum for nursery pigs. J Anim Sci. 1994. 72:2227–2236.

19. Leeds AR, Bolster NR, Andrews R, Truswell AS. Meal viscosity, gastric emptying and glucose absorption in the rat. Proc Nutr Soc. 1979. 38(2):44A.

20. Hoseney RC. Principles of cereal science and technology. 1994. St Paul, USA: American Association of cereal chemists.

21. Morrison WR, Tester RF. Properties of damage starch granules. IV. Composition of ball-milled wheat starches and of fractions obtained on hydration. J Cereal Sci. 1994. 20:69–67.

22. Martínez-Bustos F, López-Soto M, San Martín-Martínez E, Zazuet-Morales JJ, Velez-Medina JJ. Effect of high energy milling on some functional properties of jicama starch (Pachyrrhizus erosusL. Urban) and cassava starch (Manihot esculenta Crantz). J Food Engineering. 2007. 78:1212–1220.

23. Medel P, Salado S, de Blas JC, Mateos GG. Processed cereals in diets for early-weaned piglets. Anim Feed Sci Technol. 1999. 82:145–156.

24. Englyst HN, Cummings JH. Digestion of the polysaccharides of some cereal foods in the human small intestine. Am J Clin Nut. 1985. 42:778–787.

25. Morita T, Kasaoka S, Oh-hashi A, Ikai M, Numarsaki Y, Kiriyama S. Resistant proteins alter cecal short-chain fatty acid profiles in rats fed high amylose cornstarch. J Nutr. 1998. 128:1156–1164.

26. Seol SM, Bang MH, Heong MK, Kim WK. Effects of high amylose starch on gut functions in rats. Korean J Nutr. 2003. 36:10–116.

27. Bielecka M, Biedrzycka E, Majkowska A. Selection of probiotics and prebiotics for synbiotics and confirmation of their in vivo effectiveness. Food Res Int. 2002. 35:125–131.

28. Ramarkrishna BS, Venkatraman S, Srinivasan P, Dash P, Young GP, Binder HJ. Amylase-resistant starch plus oral rehydration solution for cholera. N Engl J Med. 2000. 342:308–313.

29. von Engelhardt W, Bartels J, Kirschberger S, Meyer zu Dutingdorf HD, Busche R. Role of short-chain fatty acids in the hind gut. Vet Q. 1998. 20:Suppl 3. S52–S59.

30. Mathers JC, Smith H, Cartes S. Dose-response effects of raw potato starch on small-intestinal escape, large bowel fermentation and gut transit time in the rat. Br J Nutr. 1997. 78:1015–1029.

31. Yanai T, Matsumoto C, Takashima H, Yoshida K, Sakai H, Isowa K, Iwasaki T, Sato Y, Masegi T. Immunohistochemical demonstration of S-phase cells by antibromodeoxyuridine monoclonal antibody in cattle tissues. J Comp Pathol. 1998. 114:265–272.

32. Reville M, Grosse F, Kachelhoffer J, Doffoel M, Raul F. Ileal compensation for age-dependent loss of jejunal function in rats. J Nutr. 1991. 121:498–503.

33. Sangild PT. Gut responses to enteral nutrition in preterm infants and animals. Exp Biol Med. 2006. 231:1695–1711.

34. Grand RJ, Watkins JB, Torti FM. Development of the human gastrointestinal tract: a review. Gastroenterology. 1976. 70:790–810.

35. Zabielski R, Le Huërou-Luron I, Guilloteau P. Development of gastrointestinal and pancreatic functions in mammalians (mainly bovine and porcine species): influence of age and ingested food. Reprod Nutr Dev. 1999. 39:5–26.