Anat Cell Biol.  2018 Jun;51(2):113-118. 10.5115/acb.2018.51.2.113.

Histomorphometric demonstration of the effect of chronic use of nonsteroidal anti-inflammatory drugs–ibuprofen on mucosa of small intestine

• Vaidya YP
• Tarnekar A
• Shende MR

• KMID: 2421175
• DOI: http://doi.org/10.5115/acb.2018.51.2.113

Abstract

The purpose of this study was to ascertain change in structure of mucosa of small intestine, if any, in small intestine of Swiss albino mice as an effect of chronic use of nonsteroidal anti-inflammatory drugs-Ibuprofen. Longitudinal study conducted on 46 adult Swiss albino mice, 23 as experimental and 23 as control. Ibuprofen was given at a dose of 40 µg/g body weight per day for 6 weeks by intragastric route in experimental group of mice while control group of mice received same volume of distilled water. Mice of both the groups were sacrificed and desired segments of small intestines were dissected out and tissues were subjected to histological processing. Histomorphometry was performed and relevant photomicrographs were obtained. Student's unpaired t test by GraphPad Prism 6 software. Height of villi was not significantly altered but there was significant reduction of the number of goblet and non-goblet cells (enterocytes and other columnar cells) in mucosal lining of the small intestine of experimental group of mice. Percent distribution of the goblet and non-goblet cells was not altered in villi of two groups. Chronic exposure of Ibuprofen in therapeutic dosage caused reduction of the functional cell mass in lining epithelium of villi of middle segment of small intestine. However, there was no evidence of ulcerative or hemorrhagic lesion.

Keyword

Enterocytes; Goblet cells; Ibuprofen; Morphometry; Small intestine

MeSH Terms

Adult
Animals
Body Weight
Enterocytes
Epithelium
Goblet Cells
Humans
Ibuprofen
Intestine, Small*
Longitudinal Studies
Mice
Mucous Membrane*
Ulcer
Water
Ibuprofen
Water

Figure

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  Fig. 1 Gross examination of the interior of intestinal segment: note normal appearance of plicae circulares (*) and ‘villi’ as furry coat (arrows).

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  Fig. 2 Distribution of goblet and non-goblet cells per villus (%) in control group shown by pie diagram.

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  Fig. 3 Distribution of goblet and non-goblet cells per villus (%) in experimental group shown by pie diagram.

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  Fig. 4 Section of small intestine showing distribution (arrows) of goblet cells (control group; Alcian blue, ×100).

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  Fig. 5 Section of small intestine showing distribution (arrows) of goblet cells (experimental group; Alcian blue, ×100).

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  Fig. 6 Section of small intestine showing goblet cell (G), enterocytes (E), and villus core (*) (control; H&E, 1,000).

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  Fig. 7 Section of small intestine showing goblet cells (G), enterocytes (E) on villus with villus core (*) and crypt (C) (experimental; Alcian blue, ×400).

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