J Vet Sci.  2018 Sep;19(5):643-652. 10.4142/jvs.2018.19.5.643.

Vaccination of goats with a combination Salmonella vector expressing four Brucella antigens (BLS, PrpA, Omp19, and SOD) confers protection against Brucella abortus infection

Affiliations
  • 1College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea. bskims@jbnu.ac.kr
  • 2Korea Zoonosis Research Institute, Chonbuk National University, Iksan 54596, Korea.

Abstract

Salmonella is an intracellular pathogen with a cellular infection mechanism similar to that of Brucella, making it a suitable choice for use in an anti-Brucella immune boost system. This study explores the efficacy of a Salmonella Typhimurium delivery-based combination vaccine for four heterologous Brucella antigens (Brucella lumazine synthase, proline racemase subunit A, outer-membrane protein 19, and Cu/Zn superoxide dismutase) targeting brucellosis in goats. We inoculated the attenuated Salmonella delivery-based vaccine combination subcutaneously at two different inoculation levels; 5 × 10⁹ colony-forming unit (CFU)/mL (Group B) and 5 × 10¹â° CFU/mL (Group C) and challenged the inoculations with virulent Brucella abortus at 6 weeks post-immunization. Serum immunoglobulin G titers against individual antigens in Salmonella immunized goats (Group C) were significantly higher than those of the non-immunized goats (Group A) at 3 and 6 weeks after vaccination. Upon antigenic stimulation, interferon-γ from peripheral blood mononuclear cells was significantly elevated in Groups B and C compared to that in Group A. The immunized goats had a significantly higher level of protection as demonstrated by the low bacterial loads in most tissues from the goats challenged with B. abortus. Relative real-time polymerase chain reaction results revealed that the expression of Brucella antigens was lower in spleen, kidney, and lung of immunized goats than of non-immunized animals. Also, treatment with our combination vaccine ameliorated histopathological lesions induced by the Brucella infection. Overall, the Salmonella Typhimurium delivery-based combination vaccine was effective in delivering immunogenic Brucella proteins, making it potentially useful in protecting livestock from brucellosis.

Keyword

Brucella vaccine; Salmonella vector; brucellosis; goats; protective efficacy

MeSH Terms

Animals
Bacterial Load
Brucella abortus*
Brucella Vaccine
Brucella*
Brucellosis
Goats*
Immunoglobulin G
Kidney
Livestock
Lung
Proline
Real-Time Polymerase Chain Reaction
Salmonella typhimurium
Salmonella*
Spleen
Stem Cells
Superoxides
Vaccination*
Brucella Vaccine
Immunoglobulin G
Proline
Superoxides
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