Tuberc Respir Dis.  2018 Apr;81(2):132-137. 10.4046/trd.2017.0079.

An Increased Proportion of Apoptosis in CD4⁺ T Lymphocytes Isolated from the Peripheral Blood in Patients with Stable Chronic Obstructive Pulmonary Disease

• Ju J

• KMID: 2420601
• DOI: http://doi.org/10.4046/trd.2017.0079

Abstract

BACKGROUND
The pathophysiology of chronic obstructive pulmonary disease (COPD) includes inflammation, oxidative stress, an imbalance of proteases and antiproteases and apoptosis which has been focused on lately. Abnormal apoptotic events have been demonstrated in both epithelial and endothelial cells, as well as in inflammatory cells including neutrophils and lymphocytes in the lungs of COPD patients. An increased propensity of activated T lymphocytes to undergo apoptosis has been observed in the peripheral blood of COPD patients. Therefore, the apoptosis of T lymphocytes without activating them was investigated in this study.
METHODS
Twelve control subjects, 21 stable COPD patients and 15 exacerbated COPD patients were recruited in the study. The T lymphocytes were isolated from the peripheral blood using magnetically activated cell sorting. Apoptosis of the T lymphocytes was assessed with flow cytometry using Annexin V and 7-aminoactinomycin D. Apoptosis of T lymphocytes at 24 hours after the cell culture was measured so that the T lymphocyte apoptosis among the control and the COPD patients could be compared.
RESULTS
Stable COPD patients had increased rates of CD4+ T lymphocyte apoptosis at 24 hours after the cell culture, more than the CD4+ T lymphocyte apoptosis which appeared in the control group, while the COPD patients with acute exacerbation had an amplified response of CD4+ T lymphocyte apoptosis as well as of CD8+ T lymphocyte apoptosis at 24 hours after the cell culture.
CONCLUSION
Stable COPD patients have more apoptosis of CD4+ T lymphocytes, which can be associated with the pathophysiology of COPD in stable conditions.

Keyword

Pulmonary Disease, Chronic Obstructive; T-Lymphocytes; Apoptosis

MeSH Terms

Annexin A5
Apoptosis*
Cell Culture Techniques
Endothelial Cells
Flow Cytometry
Humans
Inflammation
Lung
Lymphocytes
Neutrophils
Oxidative Stress
Peptide Hydrolases
Protease Inhibitors
Pulmonary Disease, Chronic Obstructive*
T-Lymphocytes*
Annexin A5
Peptide Hydrolases
Protease Inhibitors

Figure

• 

  Figure 1 Annexin V vs. 7-aminoactinomycin D (7AAD) staining. The percentage of Annexin V-positive/7AAD-negative cells (representing cells in the early stage of apoptosis) and Annexin V-positive/7AAD-positive cells (representing cells in the late stage of apoptosis) were measured using flow cytometry with a FACScalibur system.

• 

  Figure 2 (A) Correlation of apoptosis between CD4+ T lymphocyte and CD8+ T lymphocyte in patients with stable chronic obstructive pulmonary disease (COPD) at 0 hour. (B) Correlation of apoptosis between CD4+ T lymphocyte and CD8+ T lymphocyte in patients with stable COPD at 24 hours after culture. r=Pearson correlation coefficient, *p<0.05.

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