Korean J Parasitol.  2018 Aug;56(4):391-396. 10.3347/kjp.2018.56.4.391.

Genetic Diversity of Echinococcus granulosus Genotype G1 in Xinjiang, Northwest of China

Affiliations
  • 1School of Medicine, Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region 832002, People’s Republic of China. wangyuanzhi621@126.com
  • 2Department of Infectious Diseases, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
  • 3School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region 832002, People’s Republic of China.
  • 4Institue of Veterinary Sciences, Academy of Military Medical Sciences, Jilin, Changchun, 1300062, People’s Republic of China.
  • 5College of Animal Science, Tarim University, Alar City, Xinjiang 843300, PR China.

Abstract

Cystic echinococcosis (CE) caused by E. granulosus is a serious helminthic zoonosis in humans, livestock and wildlife. Xinjiang is one of high endemic province for CE in China. A total of 55 sheep and cattle livers containing echinococcal cysts were collected from slaughterhouses in Changji and Yining City, northern region of Xinjiang. PCR was employed for cloning 2 gene fragments, 12S rRNA and CO1 for analysis of phylogenetic diversity of E. granulosus. The results showed that all the samples collected were identified as G1 genotype of E. granulosus. Interestingly, YL5 and CJ75 strains were the older branches compared to those strains from France, Argentina, Australia. CO1 gene fragment showed 20 new genotype haploids and 5 new genotype haplogroups (H1-H5) by the analysis of Network 5.0 software, and the YLY17 strain was identified as the most ancestral haplotype. The major haplotypes, such as CJ75 and YL5 strains, showed identical to the isolates from Middle East. The international and domestic trade of livestock might contribute to the dispersal of different haplotypes for E. granulosus evolution.

Keyword

Echinococcus granulosus; CO1 gene; cystic echinococcosis; northwest China

MeSH Terms

Abattoirs
Animals
Argentina
Australia
Cattle
China*
Clone Cells
Cloning, Organism
Echinococcosis
Echinococcus granulosus*
Echinococcus*
France
Genetic Variation*
Genotype*
Haploidy
Haplotypes
Helminths
Humans
Liver
Livestock
Middle East
Polymerase Chain Reaction
Sheep
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