J Vet Sci.  2024 Jan;25(1):e7. 10.4142/jvs.22218.

G1 the common Echinococcus granulosus genotype infected domestic cat (Felis catus) in Iraq

• Al-Ardi MH

• KMID: 2553224
• DOI: http://doi.org/10.4142/jvs.22218

Abstract

Background
Infections of cats with Echinococcus granulosus is uncommon because the cat is not part of the parasite life cycle that a carnivorous and another herbivore represent. Nevertheless, it occurs incidentally when eating food or drinking water contaminated with the worm’s larva, especially with the presence of the definitive host (dogs), in this case, the infections are concentrated in stray or outside cats. For this reason, this study examined the possibility of cat infection with E. granulosus and diagnosed the common genotype of this infection.
Objective
This study examined the possibility of cat infection with E. granulosus and diagnosed the common genotype of this infection.
Methods
Four of the 37 cats that had died in different accidents developed cystic echinococcosis (CE). The cytochrome c oxidase subunit I (COX1) gene was initially amplified and sequenced to determine if these cysts belonged to E. granulosus, in beginning. The DNA fragments resulting from sequencing were then compared and aligned with other sequences using the Gene Bank database. Finally, a phylogenetic tree was drawn according to the sequence data obtained from cox1 genes sequencing, and the MEGA 7.0 phylogenetic analysis program was utilized.
Results
Four different sequences were deposited in the Gen Bank with accession numbers (ON795961 to ON795964), all of which belong to the G1 genotype. Approximately 84% and 100% of these sequences aligned with G1 (AB622277.1) and G1 (MG722980.1), respectively.
Conclusions
G1 is the dominant genotype that causes cat infections, even though the cat’s EC infection was incidental.

Keyword

Cystic echinococcosis; Echinococcus granulosus; Hydiat cysts; Felis catus; genotyping