Korean J Parasitol.
2018 Aug;56(4):371-374. 10.3347/kjp.2018.56.4.371.
Identification of Cystoisospora ohioensis in a Diarrheal Dog in Korea
- Affiliations
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- 1Banghak Animal Hospital, Seoul 01350, Korea.
- 2Pobanilab Veterinary Clinic, Guri 11906, Korea.
- 3Department of Electronic Engineering, Chosun University, Gwangju 61452, Korea.
- 4Department of Food and Nutrition, Gwangju University, Gwangju 61743, Korea.
- 5Department of Food and Nutrition, Chung-Ang University, Anseong 17546, Korea. cchoi@cau.ac.kr
- KMID: 2420322
- DOI: http://doi.org/10.3347/kjp.2018.56.4.371
Abstract
- A 3-month-old female Maltese puppy was hospitalized with persistent diarrhea in a local veterinary clinic. Blood chemistry and hematology profile were analyzed and fecal smear was examined. Diarrheal stools were examined in a diagnostic laboratory, using multiplex real-time polymerase chain reaction (PCR) against 23 diarrheal pathogens. Sequence analysis was performed using nested PCR amplicon of 18S ribosomal RNA. Coccidian oocysts were identified in the fecal smear. Although multiplex real-time PCR was positive for Cyclospora cayetanensis, the final diagnosis was Cystoisospora ohioensis infection, confirmed by phylogenetic analysis of 18S rRNA. To our knowledge, this the first case report of C. ohioensis in Korea, using microscopic examination and phylogenetic analysis.
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