Ann Dermatol.  2018 Jun;30(3):394-396. 10.5021/ad.2018.30.3.394.

Isoginkgetin Inhibits Insulin-Like Growth Factor-1-Induced Sebum Production in Cultured Human Sebocytes

• Hwang YL
• Lee MH
• Oh HI
• Kim HJ
• Lim CA
• Lee JH
• Kim CD

• KMID: 2419196
• DOI: http://doi.org/10.5021/ad.2018.30.3.394

Abstract

No abstract available.

MeSH Terms

Humans*
Sebum*

Figure

• 

  Fig. 1 (A) Structure of isoginkgetin. (B) Cytotoxicity of isoginkgetin. Sebocytes were treated with isoginkgetin at the indicated concentrations for 2 days. Cell viability was measured by MTT assay. The mean values±standard deviation are averages of triplicate measurements. (C) Effect of isoginkgetin on lipogenesis in sebocytes. Cells were treated with insulin-like growth factor-1 (IGF-1) and isoginkgetin at the indicated concentrations for 2 days. Effect of isoginkgetin on IGF-1-induced lipid production was determined by thin layer chromatography. Production of squalene and wax ester was significantly inhibited by isoginkgetin.

• 

  Fig. 2 (A) Effect of isoginkgetin on intracellular signaling. Sebocytes were pretreated with isoginkgetin at the indicated concentrations for 30 minutes, then insulin-like growth factor-1 (IGF-1) was added to the culture and incubated for a further 30 minutes. Cellular proteins were prepared and phosphorylation of signaling molecules was evaluated by Western blot. (B) Effect of isoginkgetin on the protein level for lipogenic transcription factors. Cells were treated with IGF-1 and isoginkgetin at the indicated concentrations for 2 days. Protein level for sterol response element-binding protein-1 (SREBP)-1 and peroxisome proliferator-activated receptor-γ (PPAR-γ) was evaluated by Western blot. Actin was used as a loading control.

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