J Korean Ophthalmol Soc.  2018 Aug;59(8):724-729. 10.3341/jkos.2018.59.8.724.

Anti-pseudomonal Effect of a Nephrite-containing Contact Lens Storage Case

• Lee SM
• Jung JW
• Lee DH
• Park SH
• Lee JH
• Yu HS
• Kim YK
• Lee JE

• KMID: 2418498
• DOI: http://doi.org/10.3341/jkos.2018.59.8.724

Abstract

PURPOSE
To compare the anti-pseudomonal effect of nephrite-containing contact lens (CL) storage cases with conventional CL storage cases.
METHODS
The degree of proliferation and adhesion of Pseudomonas aeruginosa (P. aeruginosa) on 1st-3rd generation silicone hydrogel contact lenses (SHCLs) was compared by using both 5% nephrite-containing CL storage cases and conventional CL storage cases. The degree of adhesion of P. aeruginosa to the CL was also compared in 1% or 5% nephrite solution and multipurpose solution (MPS).
RESULTS
Nephrite-containing CL storage cases showed a significant inhibitory effect on P. aeruginosa proliferation (p = 0.03) and significantly reduced the number of adherent P. aeruginosa on all 1st-3rd generation SHCLs compared with conventional CL storage cases (p = 0.02, p = 0.03, and p < 0.01, respectively). There was no significant anti-pseudomonal effect of 1% and 5% nephrite solutions compared with MPS.
CONCLUSIONS
Nephrite may be the next generation substance that reduces infectious keratitis caused by P. aeruginosa when added to CL storage cases.

Keyword

Contact lens storage case; Infectious keratitis; Multipurpose solution; Nephrite; Pseudomonas aeruginosa

MeSH Terms

Contact Lenses
Hydrogel
Keratitis
Pseudomonas aeruginosa
Silicon
Silicones
Hydrogel
Silicon
Silicones

Figure

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  Figure 1. Representative photographs of bacterial colonies on Luria-Bertani agar plate. The number of Pseudomonas aeruginosa colonies was significantly reduced in 5% contact lens (CL) storage cases containing 5% nephrite (B) compared with traditional CL storage cases (A).

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  Figure 2. Number of Pseudomonas aeruginosa (P. aeruginosa) on contact lens (CL) storage cases. CL storage cases containing 5% nephrite significantly reduced the numbers of P. aeruginosa compared with traditional CL storage cases. Using a unpaired t-test to compare the differences between the two groups.

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  FIgure 3. Representative photographs of bacterial colonies on Luria-Bertani agar plate. These pictures (A-F) show the decreased number of Pseudomonas aeruginosa colonies in all generations of silicone hydrogel contact lenses stored at contact lens (CL) storage cases containing 5% nephrite.

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  Figure 4. The number of Pseudomonas aeruginosa (P. aeruginosa) adhering to contact lenses. The number of P. aeruginosa adhering to contact lenses in contact lens storage case containing 5% nephrite was significantly reduced compared with tra-ditional contact lens storage case regardless of the generation of silicone hydrogel contact lenses. Using a unpaired t-test to compare the differences between the two groups.

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  Figure 5. Relationship of the number of Pseudomonas aeruginosa colonies among contact lenses treated with multipurpose solution and 1% and 5% nephrite solutions. Nephrite solutions showed a lot of colonies compared with optifree solution, regardless of the generation of silicone hydrogel contact lenses. Using a unpaired t-test to compare the differences between the two groups.

Reference

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