Tissue Eng Regen Med.
2018 Aug;15(4):437-444. 10.1007/s13770-018-0124-z.
A Pathophysiological Validation of Collagenase II-Induced Biochemical Osteoarthritis Animal Model in Rabbit
- Affiliations
-
- 1Department of Medical Engineering, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemungu, Seoul 02447, South Korea.
- 2R&D Institute, Biosolution Inc., Seoul Technopark, 232 Gongneung-ro, Nowon-gu, Seoul 01811, South Korea.
- 3Department of Orthopaedic Surgery, School of Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemungu, Seoul 02447, South Korea.
- 4Department of Biology, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, South Korea.
- 5Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yong-in, Gyeonggi-do 17104, South Korea.
- 6Impedance Imaging Research Center, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, South Korea. eunahlee@khu.ac.kr
- 7Department of Biomedical Engineering, School of Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemungu, Seoul 02447, South Korea. tioh@khu.ac.kr
- KMID: 2418100
- DOI: http://doi.org/10.1007/s13770-018-0124-z
Abstract
- BACKGROUND
Current dilemma working with surgically-induced OA (osteoarthritis) model include inconsistent pathological state due to various influence from surrounding tissues. On the contrary, biochemical induction of OA using collagenase II has several advantageous points in a sense that it does not involve surgery to induce model and the extent of induced cartilage degeneration is almost uniform. However, concerns still exists because biochemical OA model induce abrupt destruction of cartilage tissues through enzymatic digestion in a short period of time, and this might accompany systemic inflammatory response, which is rather a trait of RA (rheumatoid arthritis) than being a trait of OA.
METHODS
To clear the concern about the systemic inflammatory response that might be caused by abrupt destruction of cartilage tissue, OA was induced to only one leg of an animal and the other leg was examined to confirm the presence of systemic degenerative effect.
RESULTS
Although the cartilage tissues were rapidly degenerated during short period of time upon biochemical induction of OA, they did not accompanied with RA-like process based on the histology data showing degeneration of articular cartilage occurred only in the collagenase-injected knee joint. Scoring evaluation data indicated that the cartilage tissues in non-induced joint remained intact. Neutrophil count transiently increase between day 8 and day 16, and there were no significant change in other complete blood count profile showing a characteristics of OA disease.
CONCLUSION
These study shows that biochemically induced cartilage degeneration truly represented uniform and reliable OA state.
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