Tissue Eng Regen Med.
2018 Aug;15(4):403-413. 10.1007/s13770-018-0130-1.
Fabrication of Microchannels and Evaluation of Guided Vascularization in Biomimetic Hydrogels
- Affiliations
-
- 1Department of Biomedical Engineering, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul 02841, Republic of Korea. ydpark@kumc.or.kr
- 2Department of Mechanical Design Engineering, College of Engineering, Wonkwang University, 460 Iksandae-ro, Iksan, Jeonbuk 54538, Republic of Korea. youngsamcho@wku.ac.kr
- 3Department of Biomedical Engineering, Asan Medical Center, College of Medicine, Ulsan University, 88 Olympicro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea.
- 4Department of Oral and Maxillofacial Surgery, Asan Medical Center, College of Medicine, Ulsan University, 88 Olympicro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea.
- 5Department of Biomedical Engineering, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan 48513, Republic of Korea.
- KMID: 2418097
- DOI: http://doi.org/10.1007/s13770-018-0130-1
Abstract
- BACKGROUND
The fabrication of microchannels in hydrogel can facilitate the perfusion of nutrients and oxygen, which leads to guidance cues for vasculogenesis. Microchannel patterning in biomimetic hydrogels is a challenging issue for tissue regeneration because of the inherent low formability of hydrogels in a complex configuration. We fabricated microchannels using wire network molding and immobilized the angiogenic factors in the hydrogel and evaluated the vasculogenesis in vitro and in vivo.
METHODS
Microchannels were fabricated in a hyaluronic acid-based biomimetic hydrogel by using "wire network molding" technology. Substance P was immobilized in acrylated hyaluronic acid for angiogenic cues using Michael type addition reaction. In vitro and in vivo angiogenic activities of hydrogel with microchannels were evaluated.
RESULTS
In vitro cell culture experiment shows that cell viability in two experimental biomimetic hydrogels (with microchannels and microchannels + SP) was higher than that of a biomimetic hydrogel without microchannels (bulk group). Evaluation on differentiation of human mesenchymal stem cells (hMSCs) in biomimetic hydrogels with fabricated microchannels shows that the differentiation of hMSC into endothelial cells was significantly increased compared with that of the bulk group. In vivo angiogenesis analysis shows that thin blood vessels of approximately 25-30 µm in diameter were observed in the microchannel group and microchannel + SP group, whereas not seen in the bulk group.
CONCLUSION
The strategy of fabricating microchannels in a biomimetic hydrogel and simultaneously providing a chemical cue for angiogenesis is a promising formula for large-scale tissue regeneration.
MeSH Terms
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Angiogenesis Inducing Agents
Biomimetics*
Blood Vessels
Cell Culture Techniques
Cell Survival
Cues
Endothelial Cells
Fungi
Humans
Hyaluronic Acid
Hydrogel*
Hydrogels*
In Vitro Techniques
Mesenchymal Stromal Cells
Oxygen
Perfusion
Regeneration
Substance P
Angiogenesis Inducing Agents
Hyaluronic Acid
Hydrogel
Hydrogels
Oxygen
Substance P
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