Clin Exp Vaccine Res.  2018 Jul;7(2):129-138. 10.7774/cevr.2018.7.2.129.

Development of dual reporter imaging system for Francisella tularensis to monitor the spatio-temporal pathogenesis and vaccine efficacy

Affiliations
  • 1Department of Nuclear Medicine, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea. hwyoun@snu.ac.kr
  • 2Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.
  • 3International Vaccine Institute, Seoul, Korea.
  • 4Interpark Bio-Convergence Center, I-Market-Korea, Seoul, Korea. kevin.hong@imarketkorea.com
  • 5Cancer Imaging Center, Seoul National University Hospital, Seoul, Korea.

Abstract

PURPOSE
Study on the pathogen and the pathogen-related disease require the information at both cellular and organism level. However, lack of appropriate high-quality antibodies and the difference between the experimental animal models make it difficult to analyze in vivo mechanism of pathogen-related diseases. For more reliable research on the infection and immune-response of pathogen-related diseases, accurate analysis is essential to provide spatiotemporal information of pathogens and immune activity to avoid false-positive or mis-interpretations. In this regards, we have developed a method for tracking Francisella tularensis in the animal model without using the specific antibodies for the F. tularensis.
MATERIALS AND METHODS
A dual reporter plasmid using GFP-Lux with putative bacterioferritin promoter (pBfr) was constructed and transformed to F. tularensis live vaccine strain to generate F. tularensis LVS (FtLVS)-GFP-Lux for both fluorescence and bioluminescence imaging. For vaccination to F. tularensis infection, FtLVS and lipopolysaccharide (LPS) from FtLVS were used.
RESULTS
We visualized the bacterial replication of F. tularensis in the cells using fluorescence and bioluminescence imaging, and traced the spatio-temporal process of F. tularensis pathogenesis in mice. Vaccination with LPS purified from FtLVS greatly reduced the bacterial replication of FtLVS in animal model, and the effect of vaccination was also successfully monitored with in vivo imaging.
CONCLUSION
We successfully established dual reporter labeled F. tularensis for cellular and whole body imaging. Our simple and integrated imaging analysis system would provide useful information for in vivo analysis of F. tularensis infection as well as in vitro experiments, which have not been fully explained yet with various technical problems.

Keyword

In vivo imaging; Vaccine; Francisella tularensis

MeSH Terms

Animals
Antibodies
Fluorescence
Francisella tularensis*
Francisella*
Immunodeficiency Virus, Feline
In Vitro Techniques
Methods
Mice
Models, Animal
Plasmids
Vaccination
Whole Body Imaging
Antibodies

Figure

  • Fig. 1 Construction of a plasmid with dual reporter and generation of reporter expressing Francisella tularensis. (A) Design of a dual reporter construct. (B) Generation of reporter expressing FtLVS. (C) Comparison of reporter activity under the different promoter. (D) Plate images of FtLVS-GFP-Lux with putative Bfr promoter. RFI, relative fluorescence intensity; RLI, relative luciferase intensity; CFU, colony forming unit; BLI, bioluminescent imaging; FLI, fluorescent imaging; FtLVS, F. tularensis LVS; Bfr, bacterioferritin.

  • Fig. 2 Bioluminescence and fluorescence imaging of FtLVS-GFP-Lux and infected mouse. (A) Dose-dependent fluorescence and bioluminescence signals from FtLVS-GFP-Lux. (B) Dose-dependent fluorescent signals from FtLVS-GFP-Lux infected mouse model. Higher CFU of FtLVS-GFP-Lux infected mouse showed detectable fluorescent signal for in vivo imaging. (C) Flow cytometic analysis of cells from the organs of FtLVS-GFP-Lux infected mouse. FtLVS, Francisella tularensis LVS; CFU, colony forming unit; BLI, bioluminescent imaging; FLI, fluorescent imaging.

  • Fig. 3 Spatio-temporal imaging of FtLVS-GFP-Lux infected mouse. (A) Spatio-temporal bioluminescence imaging of FtLVS-GFP-Lux (102 CFU/mouse) infected mouse by injection route. (B) Fluorescent and luminescent signals from each organ from LVS-GFP-LUX infected mice. (C) Monitoring vaccination effects of FtLVS (1×103 CFU) in each organ after challenging with FtLVS-GFP-LUX (3 weeks after vaccination, 1×106 CFU). FtLVS, Francisella tularensis LVS; CFU, colony forming unit; FLI, fluorescent imaging; BLI, bioluminescent imaging.

  • Fig. 4 FtLVS-GFP-Lux imaging after LPS vaccination. (A) Experimental plan to evaluate FtLVS-GFP-Lux imaging system. (B) Time-lapse imaging of splenocytes from non-vaccinated mouse and LPS vaccinated mouse. (C) Flow cytometry analysis of cells from organs of non-vaccinated mouse and LPS vaccinated mouse. (D) Bacterial burden on target organ after FtLVS-GFP-Lux challenge by LPS amount of vaccination. (E) Ex-vivo bioluminescence imaging of FtLVS-GFP-Lux infected mouse by LPS amount of vaccination. (F) Whole body bioluminescence imaging of FtLVS-GFP-Lux infected mouse with or without LPS vaccination. (G) Survival rate of infected mice with or without LPS vaccination. FtLVS, Francisella tularensis LVS; LPS, lipopolysaccharide; FACS, fluorescence-activated cell sorting; PBS, phosphate buffered saline; CFU, colony forming unit.


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