Clin Exp Reprod Med.  2018 Jun;45(2):88-93. 10.5653/cerm.2018.45.2.88.

Seminal reactive oxygen species and total antioxidant capacity: Correlations with sperm parameters and impact on male infertility

• Subramanian V
• Ravichandran A
• Thiagarajan N
• Govindarajan M
• Dhandayuthapani S
• Suresh S

• KMID: 2415104
• DOI: http://doi.org/10.5653/cerm.2018.45.2.88

Abstract

OBJECTIVE
The aim of this study was to measure reactive oxygen species (ROS) production and total antioxidant capacity (TAC) in the seminal fluid of the male partners in couples undergoing intrauterine insemination and to evaluate correlations between these values and their semen parameters.
METHODS
The study was conducted at Vamsam Fertility Center, Coimbatore, India and enrolled 110 male patients from whom semen samples were collected. ROS production was measured by a thiobarbituric acid reactive species assay, and TAC was measured by a 2,2-diphenyl-2-picrylhydrazyl free radical assay. The differences in the TAC and malondialdehyde (MDA) levels between the subfertile and fertile groups were analysed. Correlations between sperm parameters and TAC and MDA levels were statistically analysed, and cutoff values with respect to the controls were determined. All hypothesis tests used were two-tailed, with statistical significance assessed at the level of p < 0.05.
RESULTS
A total of 87 subfertile and 23 fertile men were included in the study. The mean MDA level was significantly higher in the subfertile subjects than in the fertile subjects, and the mean antioxidant level was significantly lower in the subfertile subjects than in the fertile subjects. Seminal MDA levels were negatively associated with sperm concentration, motility, and morphology, whereas the opposite was seen with TAC levels.
CONCLUSION
Measurements of seminal TAC and ROS are valuable for predicting semen quality, and hence predicting the outcomes of fertility treatment.

Keyword

Male infertility; Reactive oxygen species; Sperm count; Sperm morphology; Sperm motility

MeSH Terms

Family Characteristics
Fertility
Humans
India
Infertility, Male*
Insemination
Male
Male*
Malondialdehyde
Reactive Oxygen Species*
Semen
Semen Analysis
Sperm Count
Sperm Motility
Spermatozoa*
Malondialdehyde
Reactive Oxygen Species