J Korean Assoc Oral Maxillofac Surg.  2018 Jun;44(3):120-127. 10.5125/jkaoms.2018.44.3.120.

Expression of collagenases (matrix metalloproteinase-1, 8, 13) and tissue inhibitor of metalloproteinase-1 of retrodiscal tissue in temporomandibular joint disorder patients

• Gho WG
• Choi Y
• Park KH
• Huh JK

• KMID: 2414431
• DOI: http://doi.org/10.5125/jkaoms.2018.44.3.120

Abstract

OBJECTIVES
The aim of this study was to reveal how collagenases (matrix metalloproteinase [MMP]-1, 8, 13) and tissue inhibitor of metalloproteinase 1 (TIMP-1) are expressed in immunohistochemistry of retrodiscal tissue in temporomandibular joint disorder patients.
MATERIALS AND METHODS
This study was conducted on 39 patients who underwent discoplasty or discectomy. Immunohistochemical staining was undertaken and expression levels of MMP-1, 8, 13, and TIMP-1 were evaluated. The status of internal derangement of disc, osteoarthritis, and joint effusion were analyzed using magnetic resonance imaging (MRI). Disc status observed during operation was also categorized.
RESULTS
The more severe disc derangement was observed on MRI, the more increased expression of MMPs and TIMP-1 appeared. Regarding MMP-13 expression, 86.7% of late-stage disc displacement patients showed grade II or III. Expression level of MMPs or TIMP was not statistically significant associated with joint effusion level. In perforation and/or adhesion groups, all patients showed grade II or III expression of MMP-13. Once perforation occurred, MMP-13 showed increased expression with statistical significance.
CONCLUSION
MMP-1 and MMP-13 expression seem to be related to progression of osteoarthritis whereas MMP-8 does not seem to have a specific role with regard to temporomandibular joint disorders. TIMP-1 is considered to be partly related to internal derangement rather than osteoarthritis, but it is not significant.

Keyword

Collagenases; Matrix metalloproteinase; Tissue inhibitor of metalloproteinase; Temporomandibular joint disorders; Immunohistochemistry

MeSH Terms

Collagenases*
Diskectomy
Humans
Immunohistochemistry
Joints
Magnetic Resonance Imaging
Matrix Metalloproteinases
Osteoarthritis
Temporomandibular Joint Disorders*
Temporomandibular Joint*
Tissue Inhibitor of Metalloproteinase-1*
Collagenases
Matrix Metalloproteinases
Tissue Inhibitor of Metalloproteinase-1

Figure

• 

  Fig. 1 Immunohistochemistry. A, B. Grade I expression, with extremely small portions of retrodiscal tissue removed after discoplasty was colorized (A: ×200, B: ×400). C, D. Grade II expression, with higher numbers of positive tissue cells and clearer expression than grade I (C: ×200, D: ×400). E, F. Grade III expression, with even expression in cytoplasm and broad staining (E: ×200, F: ×400).

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