J Vet Sci.  2016 Sep;17(3):385-390. 10.4142/jvs.2016.17.3.385.

Establishment of a canine mammary gland tumor cell line and characterization of its miRNA expression

• Osaki T
• Sunden Y
• Sugiyama A
• Azuma K
• Murahata Y
• Tsuka T
• Ito N
• Imagawa T
• Okamoto Y

• KMID: 2413139
• DOI: http://doi.org/10.4142/jvs.2016.17.3.385

Abstract

Canine mammary gland tumors (CMGTs), which are the most common neoplasms in sexually intact female dogs, have been suggested as a model for studying human breast cancer because of several similarities, including relative age of onset, risk factors, incidence, histological and molecular features, biological behavior, metastatic pattern, and responses to therapy. In the present study, we established a new cell line, the SNP cell line, from a CMGT. A tumor formed in each NOD.CB17-Prkdc (scid)/J mouse at the site of subcutaneous SNP cell injection. SNP cells are characterized by proliferation in a tubulopapillary pattern and are vimentin positive. Moreover, we examined miRNA expression in the cultured cells and found that the expression values of miRNA-143 and miRNA-138a showed the greatest increase and decrease, respectively, of all miRNAs observed, indicating that these miRNAs might play a significant role in the malignancy of SNP cells. Overall, the results of this study indicate that SNP cells might serve as a model for future genetic analysis and clinical treatments of human breast tumors.

Keyword

canine; cell line; mammary gland tumor; miRNA

MeSH Terms

Animals
*Cell Line, Tumor/metabolism
*Disease Models, Animal
*Dogs
Female
*Gene Expression Regulation, Neoplastic
Humans
*Mammary Neoplasms, Animal/metabolism
Mice
Mice, Inbred NOD
MicroRNAs/*genetics/metabolism
MicroRNAs

Figure

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  Fig. 1 Canine mammary gland tumor from mammary tissue, mixed breed dog. Epithelial neoplastic cells with nuclei proliferated in a tubulopapillary or trabecular pattern (arrows) in the expanded milk ducts. Irregularly sized nuclei, clarification or increased size of nucleoli, multilayered arrangement, and blood or lymphatic vessel invasion of neoplastic cells were observed. Extensive necrosis is scattered throughout the neoplastic tissue. H&E stain. Scale bars = 200 µm (A), 50 µm (B).

• 

  Fig. 2 SNP mammary gland tumor from subcutaneous tumor tissue, NOD.CB17-Prkdcscid/J mice. The circumscribed neoplastic nodules are located in the dermis and subcutaneous tissue. The tumor is composed of luminal epithelium-like cells. The neoplastic cells are arranged in a tubulopapillary fashion (arrows). The nuclei are round to ovoid and normochromatic to hyperchromatic with prominent nucleoli. Cell borders are distinct. Mitotic figures and pyknotic nuclei are common. Immunohistochemistry, positive signals for vimentin are scattered in the cytoplasm of the neoplastic cells. H&E stain. Scale bars = 500 µm (A), 50 µm (B and C).

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