J Vet Sci.  2012 Dec;13(4):433-436.

Plasma free amino acid profiles of canine mammary gland tumors

• Azuma K
• Osaki T
• Tsuka T
• Imagawa T
• Minami S
• Okamoto Y

Abstract

The purpose of this study was to elucidate the relationship between plasma free amino acid (PFAA) levels and the clinical stages of mammary gland tumors (MGT) in dogs. PFAA levels in canines with malignant mammary tumors were decreased compared to those of healthy animals. The levels of aspartate and ornithine, in the dogs with tumor metastasis were significantly decreased when compared to those of dogs that did not have metastases. Results of this study indicate that PFAA levels could be a risk factor or biomarker for canine MGT metastasis.

Keyword

dog; mammary gland tumor; plasma free amino acid

MeSH Terms

Animals
Aspartic Acid
Dogs
Mammary Glands, Human
Neoplasm Metastasis
Ornithine
Plasma
Risk Factors

Reference

1. Azuma K, Osaki T, Tsuka T, Imagawa T, Okamoto Y, Takamori Y, Minami S. Effects of oral glucosamine hydrochloride administration on plasma free amino acid concentrations in dogs. Mar Drugs. 2011. 9:712–718.

2. Bossola M, Tazza L, Luciani G. Mechanisms and treatment of anorexia in end-stage renal disease patients on hemodialysis. J Ren Nutr. 2009. 19:2–9.

3. Brosnan JT. Interorgan amino acid transport and its regulation. J Nutr. 2003. 133:6 Suppl 1. 2068S–2072S.

4. Hirayama A, Kami K, Sugimoto M, Sugawara M, Toki N, Onozuka H, Kinoshita T, Saito N, Ochiai A, Tomita M, Esumi H, Soga T. Quantitative metabolome profiling of colon and stomach cancer microenvironment by capillary electrophoresis time-of-flight mass spectrometry. Cancer Res. 2009. 69:4918–4925.

5. Kato Y, Asano K, Mogi T, Kutara K, Teshima K, Edamura K, Tsumagari S, Hasegawa A, Tanaka S. Clinical significance of circulating vascular endothelial growth factor in dogs with mammary gland tumors. J Vet Med Sci. 2007. 69:77–80.

6. Kawamura I, Moldawer LL, Keenan RA, Batist G, Bothe A Jr, Bistrian BR, Blackburn GL. Altered amino acid kinetics in rats with progressive tumor growth. Cancer Res. 1982. 42:824–829.

7. Kubota A, Meguid MM, Hitch DC. Amino acid profiles correlate diagnostically with organ site in three kinds of malignant tumors. Cancer. 1992. 69:2343–2348.

8. Kurzman ID, Gilbertson SR. Prognostic factors in canine mammary tumors. Semin Vet Med Surg (Small Anim). 1986. 1:25–32.

9. Lai HS, Lee JC, Lee PH, Wang ST, Chen WJ. Plasma free amino acid profile in cancer patients. Semin Cancer Biol. 2005. 15:267–276.

10. Maeda J, Higashiyama M, Imaizumi A, Nakayama T, Yamamoto H, Daimon T, Yamakado M, Imamura F, Kodama K. Possibility of multivariate function composed of plasma amino acid profiles as a novel screening index for non-small cell lung cancer: a case control study. BMC Cancer. 2010. 10:690.

11. Medina MA, Sánchez-Jiménez F, Márquez J, Rodríguez Quesada A, Núñez de Castro I. Relevance of glutamine metabolism to tumor cell growth. Mol Cell Biochem. 1992. 113:1–15.

12. Miyagi Y, Higashiyama M, Gochi A, Akaike M, Ishikawa T, Miura T, Saruki N, Bando E, Kimura H, Imamura F, Moriyama M, Ikeda I, Chiba A, Oshita F, Imaizumi A, Yamamoto H, Miyano H, Horimoto K, Tochikubo O, Mitsushima T, Yamakado M, Okamoto N. Plasma free amino acid profiling of five types of cancer patients and its application for early detection. PLoS One. 2011. 6:e24143.

13. Noguchi Y, Zhang QW, Sugimoto T, Furuhata Y, Sakai R, Mori M, Takahashi M, Kimura T. Network analysis of plasma and tissue amino acids and the generation of an amino index for potential diagnostic use. Am J Clin Nutr. 2006. 83:513S–519S.

14. Tecles F, Caldín M, Zanella A, Membiela F, Tvarijonaviciute A, Subiela SM, Cerón JJ. Serum acute phase protein concentrations in female dogs with mammary tumors. J Vet Diagn Invest. 2009. 21:214–219.

15. Tochikubo O, Ando T. Amino Acids and Life-style Related Diseases - Application of New Aminograms in Life Science. 2010. Tokyo: Kagawa Nutrition University Publishing Division;70–120.

16. Yamagami T, Kobayashi T, Takahashi K, Sugiyama M. Prognosis for canine malignant mammary tumors based on TNM and histologic classification. J Vet Med Sci. 1996. 58:1079–1083.