J Vet Sci.  2012 Mar;13(1):39-42. 10.4142/jvs.2012.13.1.39.

Alpha basic crystallin expression in canine mammary tumors

• Guvenc T
• Gulbahar M
• Yarim M
• Kabak YB
• Karayigit
• Sozmen M

• KMID: 1365001
• DOI: http://doi.org/10.4142/jvs.2012.13.1.39

Abstract

The aim of this study was to evaluate prognostic and/or diagnostic factors of canine mammary tumors by immunohistochemically analyzing the expression of alpha basic crystallin (alphaB-c). For this, formalin-fixed, paraffin-embedded blocks of 51 naturally-occurring canine mammary tumors (11 benign and 40 malignant) were used. Tissue from eight normal canine mammary glands were served as a control. Immunohistochemically, in the control mammary tissues, a few luminal epithelial cells were alphaB-c positive but myoepithelial cells were negative. In benign or simple type malignant tumors, alphaB-c expression was observed in luminal epithelial cells while the myoepithelial basal cells were negative. In benign or complex type malign tumors, positive staining was predominantly found in the cytoplasm of epithelial cells. Immunoreactivity of alphaB-c was also observed in neoplastic myoepithelial cells. Statistically, the number of cells immunolabeled with alphaB-c was found to be significantly different among tissues from normal canine mammary glands, benign lesions, and malignant tumors (p < 0.05). alphaB-c immunoreactivity was higher in malignant tumors than the control mammary tissues (p < 0.001). Data obtained in the current study revealed a strong association between high expression levels of alphaB-c and primary mammary gland tumors in canines.

Keyword

alphaB-crystallin; canine; mammary; neoplasms; tumors

MeSH Terms

Animals
Dog Diseases/*metabolism
Dogs
Female
Immunohistochemistry/veterinary
Logistic Models
Mammary Neoplasms, Animal/*metabolism
alpha-Crystallins/*biosynthesis

Figure

• 

  Fig. 1 (A) Some alpha basic crystallin (αB-c)-positive immunoreaction observed at the luminal epithelial cells in the control mammary tissues (arrows); myoepithelial cells were negative. (B) αB-c-immunopositive reaction of the luminal epithelial cells of the simple adenoma (arrows). (C) In the complex adenocarcinoma, positive staining was predominantly found in the cytoplasm of neoplastic epithelial cells and, to a lesser extent, in the neoplastic myoepithelial cells. (D) In carcinosarcoma, no immunopositive reaction was observed in the mesenchymal elements (me), but positive staining was seen in the neoplastic epithelial cells. Immunohistochemical staining was performed with streptavidin-biotin-peroxidase and amino ethyl carbazole chromogen. A: × 220, B and C: × 140, D: × 90.

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