Clin Exp Otorhinolaryngol.  2018 Jun;11(2):133-140. 10.21053/ceo.2017.01389.

Impact and Modulations of Peripheral and Edaphic B Cell Subpopulations in Chronic Rhinosinusitis With Nasal Polyposis

• Ickrath P
• Kleinsasser N
• Ding X
• Ginzkey C
• Beyersdorf N
• Kerkau T
• Hagen R
• Hackenberg S

• KMID: 2412671
• DOI: http://doi.org/10.21053/ceo.2017.01389

Abstract

OBJECTIVES
The pathophysiological mechanisms of chronic rhinosinusitis with nasal polyposis (CRSwNP) still are discussed controversially. Regulatory B cells (Breg) are responsible for the suppression of T cell activity: deficiencies for Breg have been demonstrated to contribute to autoimmune disorders, e.g., systemic lupus erythematosus. In order to evaluate the influence of B cell subpopulations, especially Breg, on the etiology of this disease, the aim of this study was to characterize subpopulations of peripheral and edaphic B cells in CRSwNP.
METHODS
Polypoid tissue and blood samples were collected from 10 patients undergoing paranasal sinus surgery and lymphocytes were analyzed by multicolor flow cytometry.
RESULTS
There was a significantly lower frequency of B cells in nasal polyps compared to peripheral blood mononuclear cells (PBMC) in patients with CRSwNP. Mature resting B cells were the main population within B cells in PBMC, and memory B cells in nasal polyps. Remarkably, Breg and mature B cells significantly decreased in nasal polyps compared to PBMC. Memory B cells significantly increased and represented the main subpopulation in nasal polyps in patients with CRSwNP.
CONCLUSION
In this study a detailed contemporary characterization of B cell subpopulations in patients with CRSwNP is presented. The influence of edaphic B cells could play a key role in the maintenance of this chronic infectious disease.

Keyword

Chronic Rhinosinusitis; Nasal Polyps; B Cells; Regulatory B Cells; Plasma Cells

MeSH Terms

B-Lymphocytes
B-Lymphocytes, Regulatory
Communicable Diseases
Flow Cytometry
Humans
Lupus Erythematosus, Systemic
Lymphocytes
Memory
Nasal Polyps
Plasma Cells

Figure

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  Fig. 1. (A) Detection of CD45+ lymphocytes in peripheral blood mononuclear cells (PBMC) and nasal polyps in patients with chronic rhinosinusitis with nasal polyposis (CRSwNP). (B) Separation of apoptotic cells from CD19+ lymphocytes in PBMC and nasal polyps in patients with CRSwNP. SSC-A, side scatter area.

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  Fig. 2. (A) CD19+ CD20+ B cells and CD19- CD20+ plasma cells in peripheral blood mononuclear cells (PBMC) compared to nasal polyps in patients with chronic rhinosinusitis with nasal polyposis (CRSwNP). (B) Differentiation of regulatory B cells, mature and memory B cells in PBMC and nasal polyps by gating on CD38 and CD24.

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  Fig. 3. (A) Amount of B cells in nasal polyps compared to peripheral blood mononuclear cells (PBMC) in patients with chronic rhinosinusitis with nasal polyposis (CRSwNP). (B) Regulatory B cells (Breg), mature and memory B cells compared between lymphocytes from nasal polyps and PBMC in patients with CRSwNP.

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  Fig. 4. Characterization of CD19+ CD20- CD27high HLA-DRlow plasma cells and CD19+ CD20- CD27high HLA-DRhigh plasma blasts in peripheral blood mononuclear cells (PBMC) and nasal polyps in patients with chronic rhinosinusitis with nasal polyposis.

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