J Vet Sci.  2017 Aug;18(S1):381-384. 10.4142/jvs.2017.18.S1.381.

Experimental infection of dogs with highly pathogenic avian influenza virus (H5N8)

• Yuk SS
• Lee DH
• Park JK
• Tseren-Ochir EO
• Kwon JH
• Noh JY
• Song CS

• KMID: 2412563
• DOI: http://doi.org/10.4142/jvs.2017.18.S1.381

Abstract

During the highly pathogenic avian influenza (HPAI) H5N8 virus outbreak in Korea, a dog in layer farm contaminated by H5N8 was reported seropositive for HPAI H5N8. To investigate the possibility of adaptation and transmission of HPAI H5N8 to dogs, we experimentally inoculated dogs with H5N8. Viral genes were weakly detected in nasal swabs and seroconversions in inoculated and contact dogs. Although the H5N8 virus did not induced severe clinical signs to dogs, the results suggest that surveillance of farm dogs should continue as a species in which the avian influenza virus may acquire infectivity to mammals through frequent contact with the virus.

Keyword

animal experiment; dogs; influenza A virus H5N8 subtype; transmission; virulence

MeSH Terms

Animals
Antibodies, Viral/blood
Dog Diseases/*virology
Dogs
Enzyme-Linked Immunosorbent Assay/veterinary
Hemagglutination Inhibition Tests/veterinary
*Influenza A Virus, H5N8 Subtype/pathogenicity
Orthomyxoviridae Infections/transmission/*veterinary/virology
Antibodies, Viral

Figure

• 

  Fig. 1 Body temperature after inoculation of highly pathogenic avian influenza (HPAI) H5N8 virus in dogs. Four dogs were inoculated with HPAI H5N8 virus via the nasal route, and another uninoculated four dogs were housed in the same containment cages. Mock control group dogs were caged in separate cages. Rectal body temperatures were measured at 0, 1, 3, 5, 7, 9 and 11 days post-inoculation. Mean and SD of body temperatures are plotted. ANOVA with the Tukey-Kramer post hoc test was used to analyze the body temperature results. The p values < 0.05 were considered statistically significant (a, indicates significant difference in nasal inoculation vs. mock control; b, indicates significant difference in contact exposure group vs. mock control).

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