Korean J Intern Med.  2018 May;33(3):522-531. 10.3904/kjim.2016.228.

Anti-platelet effects of epigallocatechin-3-gallate in addition to the concomitant aspirin, clopidogrel or ticagrelor treatment

• Joo HJ
• Park JY
• Hong SJ
• Kim KA
• Lee SH
• Cho JY
• Park JH
• Yu CW
• Lim DS

• KMID: 2411580
• DOI: http://doi.org/10.3904/kjim.2016.228

Abstract

BACKGROUND/AIMS
Although epigallocatechin-3-gallate (EGCG), which is found in high contents in the dried leaves of green tea, has been reported to have an anti-platelet effect, synergistic effects of EGCG in addition to current anti-platelet medications remains to be elucidated.
METHODS
Blood samples were obtained from 40 participants who took aspirin (ASA, n = 10), clopidogrel (CPD, n = 10), ticagrelor (TCG, n = 10) and no anti-platelet medication (Control, n = 10). Ex vivo platelet aggregation and adhesion under various stimulators were analyzed by multiple electrode aggregometry (MEA) and Impact-R systems. PAC-1 and P-selectin expressions in human platelets were analyzed by flow cytometry.
RESULTS
In MEA analysis, adenosine diphosphate (ADP) and thrombin receptor activating peptide (TRAP)-induced platelet aggregations were lower in the CPD and the TCG groups; arachidonic acid (AA)-induced platelet aggregation was lower in the ASA group, whereas collagen (COL)-induced platelet aggregations were comparable among four groups. EGCG significantly reduced ADP- and COL-induced platelet aggregation in dose-dependent manner (ADP, p = 0.04; COL, p < 0.01). There were no additional suppressions of platelet aggregation stimulated by AA in the ASA group, and by ADP in the CPD and TCG groups. Moreover, EGCG suppressed shear stress-induced platelet adhesion on Impact-R, and had no effect on P-selectin and PAC-1 expressions.
CONCLUSIONS
Ex vivo treatment of EGCG inhibited platelet adhesion and aggregation without changes in P-selectin and PAC-1 expression. There was no additional suppressions in platelet aggregation stimulated by AA in the ASA group and ADP in the CPD and TCG groups.

Keyword

Platelet inhibitors; Catechin; Aspirin; Clopidogrel; Ticagrelor

MeSH Terms

Adenosine Diphosphate
Arachidonic Acid
Aspirin*
Blood Platelets
Catechin
Collagen
Electrodes
Flow Cytometry
Humans
P-Selectin
Platelet Aggregation
Platelet Aggregation Inhibitors
Receptors, Thrombin
Tea
Adenosine Diphosphate
Arachidonic Acid
Aspirin
Catechin
Collagen
P-Selectin
Platelet Aggregation Inhibitors
Receptors, Thrombin
Tea