Mycobiology.
2009 Jun;37(2):94-102.
Culture Conditions for the Mycelial Growth of Ganoderma applanatum
- Affiliations
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- 1Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu 702-320, Korea. jowoosik@yahoo.co.kr
- 2Department of Chemistry, College of Natural Science, Kyeongbuk National University, Daegu 702-701, Korea.
- 3Mushroom Research Division, Department of Herbal Crop Research, National Institute of Horicultural & Herbal Science, RDA, Suwon 404-707, Korea.
- 4Rural Resources Development Institute, National Academy of Agricultural Science, RDA, Suwon 404-707, Korea.
Abstract
- Ganoderma applanatum is one of the most popular medicinal mushrooms due to the various biologically active components it produces. This study was conducted to obtain basic information regarding the mycelial culture conditions of Ganoderma applanatum. Based on the colony diameter and mycelial density, PDA, YMA and MCM media were suitable for the mycelial growth of the mushroom. The optimum temperature for mycelial growth was found to be 25~30degrees C. The optimum carbon and nitrogen sources were mannose and dextrin, respectively, and the optimum C/N ratio was 2 to 10 when 2% glucose was used. Other minor components required for the optimal growth included thiamine-HCl and biotin as vitamins, succinic acid and lactic acid as organic acids, and MgSO4.7H2O, KH2PO4 and NaCl as mineral salts.
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