Mycobiology.  2008 Mar;36(1):28-33. 10.4489/MYCO.2008.36.1.028.

Favorable Culture Conditions for Mycelial Growth of Korean Wild Strains in Ganoderma lucidum

• Jayasinghe C
• Imtiaj A
• Hur H
• Lee GW
• Lee TS
• Lee UY

• KMID: 2052884
• DOI: http://doi.org/10.4489/MYCO.2008.36.1.028

Abstract

Ganoderma lucidum (Fr.) Karst (Polyporaceae), belonging to basidiomycota, is one of the most famous medicinal mushrooms. This study was carried out to investigate favorable mycelial growth conditions, such as pH, temperature, growth media, carbon sources and nitrogen sources of Korean strains in G. lucidum. The most suitable temperature for the mycelial growth was obtained at 30degrees C. In general, optimal temperature range for the mycelial growth was found at 25~30degrees C. This Mushroom has a broad pH range (5~9) for its mycelial growth and mostly favorable growth was found at pH 5. Generally, Hamada, Glucose peptone, YM, Mushroom complete and Lilly media were the most suitable for the mycelial growth of G. lucidum. Among 10 different carbon sources, dextrin, galactose and fructose were best but the rest of other carbon sources also facilitated the growth of mycelia. The most suitable nitrogen sources were ammonium acetate, glycine, arginine and calcium nitrate, but to a certain extent, all of the supplemented nitrogen sources also stimulated the mycelial growth.

Keyword

Culture condition; Ganoderma lucidum; Media; Mycelial growth; Nutrition

MeSH Terms

Acetates
Agaricales
Arginine
Basidiomycota
Calcium
Calcium Compounds
Carbon
Fructose
Galactose
Ganoderma
Glucose
Glycine
Hydrogen-Ion Concentration
Nitrates
Nitrogen
Peptones
Quaternary Ammonium Compounds
Reishi
Acetates
Arginine
Calcium
Calcium Compounds
Carbon
Fructose
Galactose
Glucose
Glycine
Nitrates
Nitrogen
Peptones
Quaternary Ammonium Compounds