Korean J Parasitol.  2018 Feb;56(1):71-74. 10.3347/kjp.2018.56.1.71.

Serodiagnosis of Extraintestinal Amebiasis: Retrospective Evaluation of the Diagnostic Performance of the Bordier® ELISA Kit

• Beyls N
• Cognet O
• Stahl JP
• Rogeaux O
• Pelloux H

• KMID: 2408527
• DOI: http://doi.org/10.3347/kjp.2018.56.1.71

Abstract

Soluble antigens from an axenic culture of Entamoeba histolytica were used to develop a commercial ELISA kit to quantify anti-E. histolytica antibodies in sera of patients with extraintestinal amebiasis in non-endemic settings. The diagnostic specificity and sensitivity of the test were assessed retrospectively using 131 human serum samples with amoebic serologic status available. They were selected according to their results in immunofluorescence (IFAT) and were separated in 2 sample categories: 64 sera with positive results by IFAT and 67 with negative results by IFAT. The sensitivity and specificity of the ELISA kit were assessed at 95.0% and 94.0% compared to the IFAT. The test can be useful to exclude a potential diagnosis of amebiasis and could be used as a screening method since ELISA is an automated technique.

Keyword

Entamoeba histolytica; amebiasis; diagnosis; ELISA

MeSH Terms

Amebiasis*
Antibodies
Axenic Culture
Diagnosis
Entamoeba histolytica
Enzyme-Linked Immunosorbent Assay*
Fluorescent Antibody Technique
Humans
Mass Screening
Methods
Retrospective Studies*
Sensitivity and Specificity
Serologic Tests*
Antibodies