Korean J Parasitol.  2017 Dec;55(6):623-630. 10.3347/kjp.2017.55.6.623.

Development of Monoclonal Antibodies for Diagnosis of Plasmodium vivax

• Linh NT
• Park H
• Lee J
• Liu DX
• Seo GE
• Sohn HJ
• Han JH
• Han ET
• Shin HJ
• Yeo SJ

• KMID: 2403713
• DOI: http://doi.org/10.3347/kjp.2017.55.6.623

Abstract

Plasmodium lactate dehydrogenase (pLDH) is a strong target antigen for the determination of infection with Plasmodium species specifically. However, a more effective antibody is needed because of the low sensitivity of the current antibody in many immunological diagnostic assays. In this study, recombinant Plasmodium vivax LDH (PvLDH) was experimentally constructed and expressed as a native antigen to develop an effective P. vivax-specific monoclonal antibody (mAb). Two mAbs (2CF5 and 1G10) were tested using ELISA and immunofluorescence assays (IFA), as both demonstrated reactivity against pLDH antigen. Of the 2 antibodies, 2CF5 was not able to detect P. falciparum, suggesting that it might possess P. vivax-specificity. The detection limit for a pair of 2 mAbs-linked sandwich ELISA was 31.3 ng/ml of the recombinant antigen. The P. vivax-specific performance of mAbs-linked ELISA was confirmed by in vitro-cultured P. falciparum and P. vivax-infected patient blood samples. In conclusion, the 2 new antibodies possessed the potential to detect P. vivax and will be useful in immunoassay.

Keyword

Plasmodium vivax; Plasmodium lactate dehydrogenase; monoclonal antibody

MeSH Terms

Antibodies
Antibodies, Monoclonal*
Diagnosis*
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
Humans
Immunoassay
L-Lactate Dehydrogenase
Limit of Detection
Plasmodium vivax*
Plasmodium*
Antibodies
Antibodies, Monoclonal
L-Lactate Dehydrogenase