Ann Surg Treat Res.  2018 Feb;94(2):57-62. 10.4174/astr.2018.94.2.57.

The effect of mesenchymal stem cell use on intra-abdominal adhesions in a rat model

Affiliations
  • 1Department of General Surgery, Faculty of Medicine, Kırıkkale University, Kırıkkale, Turkey. gokhankaracaa@yahoo.com
  • 2Department of Biochemistry, Istanbul University, Istanbul, Turkey.
  • 3Department of General Surgery, Hacettepe University, School of Medicine, Ankara, Turkey.
  • 4Department of General Surgery, Alaca State Hospital, Çorum, Turkey.
  • 5Department of Biochemistry, Bezmialem Vakif University Medical Faculty, Istanbul, Turkey.

Abstract

PURPOSE
Intra-abdominal adhesions (IAA) are among the most frequently seen pathologies in general surgery practice with an increased morbidity and mortality. In the present study, we investigated the effect of locally applied mesenchymal stem cells (MSCs) on IAA.
METHODS
Twenty-four Wistar Albino rats were used in the study. The rats were divided into three groups including: Sham, control, and MSCs group. On day 0, cecum was reached under anesthesia in all groups, except the Sham group. Scraping with a sponge was performed until petechial bleeding occurred. The control group received no treatment. In the stem cell group, MSCs were applied topically immediately after surgery on adhesions. The rats were sacrificed on day 10 and colon tissues and blood samples were collected for macroscopic, histopathological, and biochemical analysis.
RESULTS
In our study, E-selectin, P-selectin, TNF-α and IL-1 levels were statistically significantly lower in the MSC group than the control group, while the sham group has the lowest levels. In both the macroscopic and histopathological analyses (Zühlke's scale), the least amount of adhesion was observed in the Sham group. In addition, although there was less adhesion in the MSC group than the control group, the difference did not reach statistical significance.
CONCLUSION
Topical MSC application immediately after surgery suppresses the inflammatory process. However it was found to be ineffective in histopathological and macroscopic examinations performed on the 10th day.

Keyword

Mesenchymal stem cell; Adhesions; Morphological and microscopic findings; Selectins

MeSH Terms

Anesthesia
Animals
Cecum
Colon
E-Selectin
Hemorrhage
Interleukin-1
Mesenchymal Stromal Cells*
Models, Animal*
Morphological and Microscopic Findings
Mortality
P-Selectin
Pathology
Porifera
Rats*
Selectins
Stem Cells
E-Selectin
Interleukin-1
P-Selectin
Selectins
Full Text Links
  • ASTR
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error