J Vet Sci.  2018 Jan;19(1):151-155. 10.4142/jvs.2018.19.1.151.

Serological and molecular epidemiology of Japanese encephalitis virus infections in swine herds in China, 2006–2012

• Chai C
• Wang Q
• Cao S
• Zhao Q
• Wen Y
• Huang X
• Wen X
• Yan Q
• Ma X
• Wu R

• KMID: 2402706
• DOI: http://doi.org/10.4142/jvs.2018.19.1.151

Abstract

Japanese encephalitis virus (JEV) is a mosquito-borne, zoonotic flavivirus causing viral encephalitis in humans and reproductive disorder in swine. JEV is prevalent throughout China in human; however, spatiotemporal analysis of JEV in Chinese swine herds has not been reported previously. Herein, we present serological and molecular epidemiological results and estimates of prevalence of JEV infections among swine herds in various regions of China. The results suggest that JEV infections are widespread and genotype I and III strains co-exist in the same regions. Therefore, there is an urgent need to monitor JEV infection status among swine herds in China.

Keyword

China; Japanese encephalitis virus; molecular epidemiology; serological detection; swine

MeSH Terms

Asian Continental Ancestry Group*
China*
Encephalitis Virus, Japanese*
Encephalitis, Japanese*
Encephalitis, Viral
Flavivirus
Genotype
Humans
Molecular Epidemiology*
Prevalence
Spatio-Temporal Analysis
Swine*

Figure

• 

  Fig. 1 The prevalence and distribution of Japanese encephalitis virus (JEV) in swine herds in China, 2006 to 2012. Serum samples were collected from swine herds in China, thereby providing JEV epidemiological data for different regions and provinces (A), years (B), and months (C).

• 

  Fig. 2 Results of phylogenetic analysis of Japanese encephalitis virus strains isolated from Chinese swine herds based on assessment of the capsid/premembrane protein (C/PrM) nucleotide sequences. The multiple sequence alignments were obtained by using MEGA software (ver. 5.0; Molecular Evolutionary Genetics Analysis). The tree was constructed by applying the neighbor-joining method. Scale bar indicates number of nucleotide substitutions per site. Bootstrap confidence limits are shown at each node. The Dengue 1 virus (strain GZ2002) was used as an outlier group. Those strains (marked by ▲ or ●) were isolated and identified from pigs between 2006 and 2012 in China.

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