Mycobiology.
2017 Dec;45(4):434-437. 10.5941/MYCO.2017.45.4.434.
An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides
- Affiliations
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- 1College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. aiminma@mail.hzau.edu.cn
- 2College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
- 3Key Laboratory of Agro-Microbial Resources and Utilization, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China.
- KMID: 2401076
- DOI: http://doi.org/10.5941/MYCO.2017.45.4.434
Abstract
- An improved method for extracting high quality and quantity RNA from a jelly mushroom and a dimorphic fungus"”Tremella fuciformis which is especially rich in polysaccharides, is described. RNA was extracted from T. fuciformis mycelium M1332 and its parental monokaryotic yeast-like cells Y13 and Y32. The A260/280 and A260/230 ratios were both approximately 2, and the RNA integrity number was larger than 8.9. The yields of RNA were between 108 and 213 µg/g fresh wt. Downstream molecular applications including reverse transcriptional PCR and quantitative real-time PCR were also performed. This protocol is reliable and may be widely applicable for total RNA extraction from other jelly mushrooms or filamentous fungi rich in polysaccharides.
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