J Cancer Prev.  2017 Jun;22(2):115-125. 10.15430/JCP.2017.22.2.115.

Analysis of Gastric Body Microbiota by Pyrosequencing: Possible Role of Bacteria Other Than Helicobacter pylori in the Gastric Carcinogenesis

• Sohn SH
• Kim N
• Jo HJ
• Kim J
• Park JH
• Nam RH
• Seok YJ
• Kim YR
• Lee DH

• KMID: 2400776
• DOI: http://doi.org/10.15430/JCP.2017.22.2.115

Abstract

BACKGROUND
Gastric microbiota along with Helicobacter pylori (HP) plays a key role in gastric disease. The aim of our study is to investigate the difference of human gastric microbiota between antrum and body according to disease (control vs. gastric cancer) and HP status.
METHODS
Each antrum and body biopsy was collected from 12 subjects at Seoul National University Bundang Hospital. Gastric microbiota was analyzed by bar-coded 454 pyrosequencing of the 16S rRNA gene. Twelve subjects consisted of HP-negative control (n = 2), HP-negative cancer (n = 2), HP-positive control (n = 3), and HP-positive cancer (n = 5). The analysis was focused on non-HP urease-producing bacteria (UB) and non-HP nitrosating or nitroreducing bacteria (NB) between antrum and body.
RESULTS
Gastric body samples showed higher diversity compared to gastric antrum mucosa samples but there was no significant difference. The mean of operational taxonomic units was higher in HP(−) cancer than HP(+) cancer (antrum, 273.5 vs. 228.2, P = 0.439; body, 585.5 vs. 183.2, P = 0.053). The number of non-HP UB and non-HP NB was higher in HP(−) cancer groups than the others. These differences were more pronounced in the body (P = 0.051 and P = 0.081, respectively). Analysis of overlap of non-HP UB and non-HP NB revealed the higher composition of Streptococcus pseudopneumoniae, S. parasanguinis, and S. oralis in HP(−) cancer groups than the others, only in the body (P = 0.030) but not in the antrum (P = 0.123).
CONCLUSIONS
Higher diversity and higher composition of S. pseudopneumoniae, S. parasanguinis, and S. oralis in HP(−) cancer group than the other groups in the body suggest that analysis of microbiota from body mucosa could be beneficial to identify a role of non-HP bacteria in the gastric carcinogenesis.

Keyword

Microbiota; Helicobacter pylori; Antrum mucosa; Body mucosa

MeSH Terms

Bacteria*
Biopsy
Carcinogenesis*
Genes, rRNA
Helicobacter pylori*
Helicobacter*
Humans
Microbiota*
Mucous Membrane
Pyloric Antrum
Seoul
Stomach Diseases
Streptococcus