Anat Cell Biol.  2017 Dec;50(4):275-283. 10.5115/acb.2017.50.4.275.

Ameliorative effects of type-A procyanidins polyphenols from cinnamon bark in compound 48/80-induced mast cell degranulation

• Kandhare A
• Aswar UM
• Mohan V
• Thakurdesai PA

• KMID: 2399896
• DOI: http://doi.org/10.5115/acb.2017.50.4.275

Abstract

Allergic diseases are a significant health concern in developing countries. Type-A procyanidin polyphenols from cinnamon (Cinnamomum zeylanicum Blume) bark (TAPP-CZ) possesses antiasthmatic and antiallergic potential. The present study was aimed at the possible anti-allergic mechanism of TAPP-CZ against the compound 48/80 (C48/80)-induced mast cell degranulation in isolated rat peritoneal mast cells (RPMCs). TAPP-CZ (1, 3, 10, and 30 µg/ml) was incubated for 3 hours with isolated, purified RPMCs. The C48/80 (1 µg/ml) was used to induce mast cell degranulation. The mast cell viability was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay whereas histamine, β-hexosaminidase (β-HEX), and interleukin-4 (IL-4) levels were determined in RPMCs. TAPP-CZ (3, 10, and 30 µg/ml) showed significant and dose-dependent decrease in a number of degranulated cells and levels of markers (histamine, β-HEX, and IL-4) as compared with C48/80 control. In conclusion, TAPP-CZ stabilizes mast cell and cause inhibition of the allergic markers such as histamine, IL-4, and β-HEX in IgE-mediated manner. The present study supports mast cell stabilization as a possible mechanism of action of TAPP-CZ against immune respiratory disorders such as asthma and allergic rhinitis.

Keyword

Cinnamomum zeylanicum; Type-A procyanidin polyphenols; Mast cell degranulation inhibition

MeSH Terms

Animals
Asthma
Cinnamomum zeylanicum*
Developing Countries
Histamine
Interleukin-4
Mast Cells*
Polyphenols*
Proanthocyanidins*
Rats
Rhinitis, Allergic
Histamine
Interleukin-4
Polyphenols
Proanthocyanidins

Figure

• 

  Fig. 1 Light microphotographs using inverted microscopy of rat peritoneal mast cells (RPMCs) in hydroxyethyl piperazineethanesulfonic acid-Tyrode buffer. RPMCs of normal group (A), RPMCs stimulated with compound 48/80 (B), RPMCs pretreated with disodium cromoglycate (100 µg/ml) (C), RPMCs pretreated with type-A procyanidine polyphenols from Cinnamomum zeylanicum (TAPP-CZ; 3 µg/ml) (D), RPMCs pretreated with TAPP-CZ (10 µg/ml) (E), and RPMCs pretreated with TAPP-CZ (30 µg/ml) (F). White arrows indicate mast cells.

• 

  Fig. 2 Effect of type-A procyanidine polyphenols from Cinnamomum zeylanicum (TAPP-CZ) on compound 48/80 (C48/80)-induced histamine (A), β-hexosaminidase (β-HEX) (B), and interleukin 4 (IL-4) (C) release in rat peritoneal mast cells. Data was analyzed by one-way ANOVA followed by Dunnett's test. *P<0.05, **P<0.01, ***P<0.001 as compared to C48/80 control group and ###P<0.001 as compared to normal group. DSCG, disodium cromoglycate.

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