J Korean Med Sci.  2017 Dec;32(12):1997-2004. 10.3346/jkms.2017.32.12.1997.

Genetic Contributions to Childhood Obesity: Association of Candidate Gene Polymorphisms and Overweight/Obesity in Korean Preschool Children

• Yoo KH
• Yim HE
• Bae ES
• Hong YS

• KMID: 2396377
• DOI: http://doi.org/10.3346/jkms.2017.32.12.1997

Abstract

This study was aimed to investigate the association of candidate gene polymorphisms and obesity or overweight in young Korean children. A total of 190 Korean preschool children (96 control, 48 overweight, and 46 obese children) were genotyped for the angiotensin converting enzyme (ACE) insertion (I)/deletion (D), angiotensin II type 2 receptor (AT2) C3123A, transforming growth factor (TGF)-β1 T869C, vascular endothelial growth factor (VEGF) T460C, and tumor necrosis factor (TNF)-α G308A polymorphisms. No differences were found among the groups with respect to age, sex, birth weight, blood pressure levels, and serum concentrations of glucose and total cholesterol. Obese children showed a higher incidence of ACE DD genotype and D allelic frequency compared to the controls (odds ratio [OR], 2.7, 95% confidence interval [CI], 1.01-7.21; OR, 2.5, 95% CI, 1.49-4.19; all P < 0.05). The frequency of TC genotype and C allele in the TGF-β1 T869C polymorphism (OR, 2.08, 95% CI, 1.01-4.27; OR, 1.93, 95% CI, 1.15-3.21) and that in the VEGF T460C polymorphism (OR, 2.5, 95% CI, 1.19-5.28; OR, 2.15, 95% CI, 1.26-3.68) was also higher in obese children than in control subjects (all P < 0.05). Overweight children exhibited a higher frequency of the A allele in the AT2 C3123A polymorphism compared to the controls (OR, 1.72, 95% CI, 1.03-2.88, P < 0.05). There were no differences in the TNF-α G308A polymorphism among the groups. The ACE I/D, AT2 C3123A, TGF-β1 T869C, and VEGF T460C polymorphisms can affect susceptibility to obesity or overweight in Korean children.

Keyword

Angiogenic Proteins; Child Obesity; Genetic Variation; Renin Angiotensin System

MeSH Terms

Alleles
Angiogenic Proteins
Birth Weight
Blood Pressure
Child
Child, Preschool*
Cholesterol
Genetic Variation
Genotype
Glucose
Humans
Incidence
Obesity
Overweight
Pediatric Obesity*
Peptidyl-Dipeptidase A
Receptor, Angiotensin, Type 2
Renin-Angiotensin System
Transforming Growth Factors
Tumor Necrosis Factor-alpha
Vascular Endothelial Growth Factor A
Angiogenic Proteins
Cholesterol
Glucose
Peptidyl-Dipeptidase A
Receptor, Angiotensin, Type 2
Transforming Growth Factors
Tumor Necrosis Factor-alpha
Vascular Endothelial Growth Factor A

Figure

• 

  Fig. 1 Determination of genotypes using PCR amplification products. (A) ACE I/D polymorphism. (B) AT2 C3123A polymorphism. (C) TGF-β1 T869C polymorphism. (D) VEGF T460C polymorphism. (E) TNF-α G308A polymorphism. PCR = polymerase chain reaction, ACE I/D = angiotensin converting enzyme insertion/deletion, AT2 = angiotensin II type 2 receptor, TGF-β1 = transforming growth factor-β1, VEGF = vascular endothelial growth factor, TNF-α = tumor necrosis factor-α, M = marker, bp = base pairs.

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