Yonsei Med J.  2009 Apr;50(2):189-194.

Comparison of the Effect of Vitamin K2 and Risedronate on Trabecular Bone in Glucocorticoid-Treated Rats: A Bone Histomorphometry Study

• Iwamoto J
• Matsumoto
• Tadeda T
• Sato Y
• Yeh JK

Abstract

PURPOSE
To compare the effect of vitamin K2 and risedronate on trabecular bone in glucocorticoid (GC)-treated rats. MATERIALS AND METHODS: Forty-eight Sprague-Dawley female rats, 3 months of age, were randomized by the stratified weight method into 5 groups according to the following treatment schedule: age-matched control, GC administration, and GC administration with concomitant administration of vitamin K2, risedronate, or vitamin K2 + risedronate. GC (methylprednisolone sodium succinate, 5.0 mg/kg) and risedronate (10 microgram/kg) were administered subcutaneously three and five times a week, respectively. Vitamin K2 (menatetrenone, 30 mg/kg) was administered orally three times a week. At the end of the 8-week experiment, bone histomorphometric analysis was performed on trabecular bone of the tibial proximal metaphysis. RESULTS: GC administration decreased trabecular bone mass compared with age-matched controls because of decreased bone formation (mineralizing surface, mineral apposition rate, and bone formation rate) and increased bone erosion. Vitamin K2 attenuated GC-induced trabecular bone loss by preventing GC-induced decrease in bone formation (mineralizing surface) and subsequently reducing GC-induced increase in bone erosion. Risedronate prevented GC-induced trabecular bone loss by preventing GC-induced increase in bone erosion although it also suppressed bone formation (mineralizing surface, mineral apposition rate, and bone formation rate). Vitamin K2 mildly attenuated suppression of bone formation (mineralizing surface) and bone erosion caused by risedronate without affecting trabecular bone mass when administered in combination. CONCLUSION: The present study showed differential effect of vitamin K2 and risedronate on trabecular bone in GC-treated rats.

Keyword

Glucocorticoid; trabecular bone; bone histomorphometry; risedronate; vitamin K2

MeSH Terms

Animals
Bone Density/drug effects
Bone and Bones/anatomy & histology/*drug effects/metabolism
Etidronic Acid/*analogs & derivatives/pharmacology
Female
Glucocorticoids/*pharmacology
Random Allocation
Rats
Vitamin K/*pharmacology
Vitamins/*pharmacology

Figure

• 

  Fig. 1 Images of the proximal tibial metaphysis. The images of the proximal tibial metaphysis confirm the results of bone histomorphometric analysis regarding trabecular BV/TV. GC, glucocorticoid; BV, bone volume; TV, total tissue volume.

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