Ann Dermatol.  2017 Apr;29(2):149-155. 10.5021/ad.2017.29.2.149.

Low-Level Light Therapy with 410 nm Light Emitting Diode Suppresses Collagen Synthesis in Human Keloid Fibroblasts: An In Vitro Study

• Lee HS
• Jung SE
• Kim SK
• Kim YS
• Sohn S
• Kim YC

• KMID: 2394837
• DOI: http://doi.org/10.5021/ad.2017.29.2.149

Abstract

BACKGROUND
Keloids are characterized by excessive collagen deposition in the dermis, in which transforming growth factor β (TGF-β)/Smad signaling plays an important role. Low-level light therapy (LLLT) is reported as effective in preventing keloids in clinical reports, recently. To date, studies investigating the effect of LLLT on keloid fibroblasts are extremely rare.
OBJECTIVE
We investigated the effect of LLLT with blue (410 nm), red (630 nm), and infrared (830 nm) light on the collagen synthesis in keloid fibroblasts.
METHODS
Keloid fibroblasts were isolated from keloid-revision surgery samples and irradiated using 410-, 630-, 830-nm light emitting diode twice, with a 24-hour interval at 10 J/cm². After irradiation, cells were incubated for 24 and 48 hours and real-time quantitative reverse transcription polymerase chain reaction was performed. Western blot analysis was also performed in 48 hours after last irradiation. The genes and proteins of collagen type I, TGF-β1, Smad3, and Smad7 were analyzed.
RESULTS
We observed no statistically significant change in the viability of keloid fibroblasts after irradiation. Collagen type I was the only gene whose expression significantly decreased after irradiation at 410 nm when compared to the non-irradiated control. Western blot analysis showed that LLLT at 410 nm lowered the protein levels of collagen type I compared to the control.
CONCLUSION
LLLT at 410 nm decreased the expression of collagen type I in keloid fibroblasts and might be effective in preventing keloid formation in their initial stage.

Keyword

Collagen type I; Keloid fibroblast; Low-level light therapy

MeSH Terms

Blotting, Western
Collagen Type I
Collagen*
Dermis
Fibroblasts*
Humans*
In Vitro Techniques*
Keloid*
Low-Level Light Therapy*
Polymerase Chain Reaction
Reverse Transcription
Transforming Growth Factors
Collagen
Collagen Type I
Transforming Growth Factors

Figure

• 

  Fig. 1 Viable keloid fibroblasts had tendency to decrease after irradiation with all wavelengths compared to non-irradiated keloid control. However, there was no statistically significant decrease compared to the control. The results are represented mean±standard error from two independent experiments with two set of fibroblasts.

• 

  Fig. 2 Only expression of collagen type I showed a significant decrease in cell irradiate with 410 nm light compared to non-irradiated control after 48 hours. The results are represented as mean±standard error from five independent experiments. TGF-β1: transforming growth factor β1. *p<0.05 vs. non-irradiated control.

• 

  Fig. 3 Low-level light therapy suppressed the expression of collagen type I in keloid fibroblasts irradiated with 410 nm light. TGF-β1: transforming growth factor β1.

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