J Vet Sci.  2012 Dec;13(4):345-353.

Molecular characterization of the feline T-cell receptor gamma alternate reading frame protein (TARP) ortholog

Affiliations
  • 1Institute of Veterinary Pathology, Justus-Liebig-Universitat Giessen, 35392 Giessen, Germany. alexander.weiss@cvua-mel.de
  • 2Department of Veterinary Pathology, Freie Universitat Berlin, 14163 Berlin, Germany.

Abstract

T-cell receptor gamma alternate reading frame protein (TARP) is expressed by human prostate epithelial, prostate cancer, and mammary cancer cells, but is not found in normal mammary tissue. To date, this protein has only been described in humans. Additionally, no animal model has been established to investigate the potential merits of TARP as tumor marker or a target for adoptive tumor immunotherapy. In this study conducted to characterize feline T-cell receptor gamma sequences, constructs very similar to human TARP transcripts were obtained by RACE from the spleen and prostate gland of cats. Transcription of TARP in normal, hyperplastic, and neoplastic feline mammary tissues was evaluated by conventional RT-PCR. In felines similarly to the situation reported in humans, a C-region encoding two open reading frames is spliced to a J-region gene. In contrast to humans, the feline J-region gene was found to be a pseudogene containing a deletion within its recombination signal sequence. Our findings demonstrated that the feline TARP ortholog is transcribed in the prostate gland and mammary tumors but not normal mammary tissues as is the case with human TARP.

Keyword

cat; C-region; J-region; T-cell receptor gamma alternate reading frame protein (TARP); tumor marker

MeSH Terms

Animals
Cats
Continental Population Groups
Humans
Immunotherapy
Models, Animal
Open Reading Frames
Prostate
Prostatic Neoplasms
Protein Sorting Signals
Pseudogenes
Reading Frames
Receptors, Antigen, T-Cell
Recombination, Genetic
Spleen
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