Anat Cell Biol.  2017 Sep;50(3):214-218. 10.5115/acb.2017.50.3.214.

Expression of glucose transporters in the developing rat skin

• Oh CS
• Hong JH
• Jin SN
• Lee WJ
• Lee YS
• Lee E

• KMID: 2390483
• DOI: http://doi.org/10.5115/acb.2017.50.3.214

Abstract

We found the changed distribution of glucose transporter (GLUT) proteins in the skin during rat development. At 15 days of gestation, GLUT1 and 2 proteins were expressed in the stratum corneum of epidermal cells. In postnatal skin, however, GLUT1 and 2 exhibit different expression patterns. While GLUT1 expression becomes more restricted to the stratum basale with development, GLUT2 was found mainly in stratum spinosum and granulosum, but not being localized in the stratum basale at any stages of perinatal skin development. Considering all these, it can be speculated that each GLUT protein plays its specific role in different epidermal layers and that the glucose used in mammalian skin in utero could be originated from the amniotic fluid during skin development.

Keyword

Glucose transporter; Skin; Epidermis; Rat; Immunohistochemistry

MeSH Terms

Amniotic Fluid
Animals
Epidermis
Female
Glucose Transport Proteins, Facilitative
Glucose*
Immunohistochemistry
Pregnancy
Rats*
Skin*
Glucose
Glucose Transport Proteins, Facilitative

Figure

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  Fig. 1 Glucose transporter (GLUT) 1 immunoreactivity (IR) at gestational day (G) 15, G18, postnatal day (P) 0 and P7. (A, D, G, J) Image of GLUT1 IRs. (B, E, H, K) Merged image of GLUT1 IRs and DAPI. (C, F, I, L) Magnified image of merged one. EP, epidermis; D, dermis. Asterisks indicate cells of stratum basale. At G15, GLUT1 IRs were found in most epidermal cells. In magnified image of G15, GLUT1 IRs were localized in every cytoplasmic domain (epical, lateral, and basal) of the cells. After G18, the GLUT1 expression was changed. GLUT1 IRs became mainly restricted to the stratum basale of epidermis. P0 and P7 images also showed the same pattern. Scale bars=40 µm (A–L).

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  Fig. 2 Glucose transporter (GLUT) 2 immunoreactivity (IR) at gestational day (G) 15, G18, postnatal day (P) 0 and P7. (A, D, G, J) Image of GLUT2 IRs. (B, E, H, K) Merged image of GLUT2 IRs and DAPI. (C, F, I, L) Magnified image of merged one. EP, epidermis; D, dermis. Asterisks indicate cells of stratum basale. At G15, GLUT2 were localized in every cytoplasmic domain of most epidermal cells. In magnified image, the cells of stratum basale were not immune-stained by GLUT2 antibody. The absence of GLUT2 IRs in stratum basale continued even at G18, P0 and P7: the cells of stratum basale did not express GLUT2 IRs. Scale bars=40 µm (A–L).

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