Investig Clin Urol.  2017 May;58(3):210-216. 10.4111/icu.2017.58.3.210.

Antiurolithic effect of olive oil in a mouse model of ethylene glycol-induced urolithiasis

• Alenzi M
• Rahiman S
• Tantry BA

• KMID: 2388067
• DOI: http://doi.org/10.4111/icu.2017.58.3.210

Abstract

PURPOSE
At present, commercially available antiurolithic drugs have more adverse effects than potential therapeutic or preventive effects with chronic use. With this in mind, the present study was designed to assess the antiurolithic effect of olive oil in a mouse model of ethylene glycol (EG)-induced urolithiasis.
MATERIALS AND METHODS
Adult albino mice were divided into 6 groups. Group I was fed the vehicle only. Group II was supplemented with 0.75% EG alone in drinking water during the experimental period to initiate deposition of calcium oxalate in kidneys, which leads to urolithiasis in animals. Groups III (olive oil control group) through V were fed olive oil orally at various doses during the experimental period. Group VI received cystone (750 mg/kg). Groups IV-VI additionally received 0.75% EG in drinking water ad libitum. SPSS ver.17.0 was used for statistical analysis.
RESULTS
The study results showed significantly higher levels of serum urea, uric acid, and creatinine (p<0.05) in group II than in groups III-VI and I. Administration of olive oil at different doses restored the elevated serum parameters in groups IV and V compared with group II. Urine and kidney calcium, oxalate, and phosphate levels in groups IV-VI were significantly lower (p<0.05) than in animals with EG-induced urolithiasis (group II). Group V mice showed a significant restoration effect on serum as well as urine and kidney parameters compared with group II.
CONCLUSIONS
Supplementation with olive oil (1.7 mL/kg body weight) reduced and prevented the growth of urinary stones, possibly by inhibiting renal tubular membrane damage due to peroxidative stress induced by hyperoxaluria.

Keyword

Calcium oxalate; Hyperoxaluria; Kidney calculi; Olive oil; Urolithiasis

MeSH Terms

Adult
Animals
Calcium
Calcium Oxalate
Creatinine
Drinking Water
Ethylene Glycol
Humans
Hyperoxaluria
Kidney
Kidney Calculi
Membranes
Mice*
Olea*
Olive Oil*
Urea
Uric Acid
Urinary Calculi
Urolithiasis*
Calcium
Calcium Oxalate
Creatinine
Drinking Water
Ethylene Glycol
Olive Oil
Urea
Uric Acid

Figure

• 

  Fig. 1 (A) Effect of olive oil on urine calcium levels in mice with ethylene glycol-induced urolithiasis. (B) Effect of olive oil on urine oxalate levels in mice with ethylene glycol-induced urolithiasis. (C) Effect of olive oil on urine inorganic phosphate levels in mice with ethylene glycol-induced urolithiasis. Group I, vehicle only; group II, ethylene glycol only; group III, olive oil only; group IV, ethylene glycol + olive oil low quantity; group V, ethylene glycol + olive oil high quantity; group VI, ethylene glycol + cystone.

• 

  Fig. 2 (A) Effect of olive oil on kidney oxalate levels in mice with ethylene glycol-induced urolithiasis. (B) Effect of olive oil on kidney calcium levels in mice with ethylene glycol-induced urolithiasis. (C) Effect of olive oil on kidney inorganic phosphate levels in mice with ethylene glycol-induced urolithiasis. Group I, vehicle only; group II, ethylene glycol only; group III, olive oil only; group IV, ethylene glycol + olive oil low quantity; group V, ethylene glycol + olive oil high quantity; group VI, ethylene glycol + cystone.

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