Mycobiology.  2017 Jun;45(2):90-96. 10.5941/MYCO.2017.45.2.90.

Optimization of Submerged Fermentation Medium for Matrine Production by Aspergillus terreus, an Endophytic Fungus Harboring Seeds of Sophora flavescens, Using Response Surface Methodology

• Zhang Q
• Li Y
• Xu F
• Zheng M
• Xi X
• Zhang X
• Han C

• KMID: 2385656
• DOI: http://doi.org/10.5941/MYCO.2017.45.2.90

Abstract

Different endophytes isolated from the seeds of Sophora flavescens were tested for their ability to produce matrine production. Response surface methodology (RSM) was applied to optimize the medium components for the endophytic fungus. Results indicated that endophyte Aspergillus terreus had the ability to produce matrine. The single factor tests demonstrated that potato starch was the best carbon source and the combination of peptone and NHâ‚„NO₃ was the optimal nitrogen source for A. terreus. The model of RSM predicted to gain the maximal matrine production at 20.67 µg/L, when the potato starch was 160.68 g/L, peptone was 24.96 g/L and NHâ‚„NO₃ was 2.11 g/L. When cultured in the optimal medium, the matrine yield was an average of 20.63 ± 0.11 µg/L, which was consistent with the model prediction. This study offered an alternative source for the matrine production by endophytic fungus fermentation and may have far-reaching prospect and value.

Keyword

Endophytic fungi; Matrine; Response surface methodology; Sophora flavescens

MeSH Terms

Aspergillus*
Carbon
Endophytes
Fermentation*
Fungi*
Nitrogen
Peptones
Solanum tuberosum
Sophora*
Starch
Carbon
Nitrogen
Peptones
Starch