J Korean Dent Sci.  2016 Jun;9(1):1-8. 10.5856/JKDS.2016.9.1.1.

Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0℃/2 MPa Condition up to One Week: In Vivo MTT Method

• Jang SM
• Cho SY
• Kim ES
• Jung IY
• Lee SJ

• KMID: 2382972
• DOI: http://doi.org/10.5856/JKDS.2016.9.1.1

Abstract

PURPOSE
The aim of this study was to evaluate the rat periodontal ligament cell viability under 0℃/2 MPa condition up to one week using in vivo 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay.
MATERIALS AND METHODS
As soon as 110 upper molar teeth of rats were extracted, they were stored in Hartman's solution under 0℃/2 MPa condition for 1, 2, 3, 4, and 7 days each. All specimens were treated with in vivo MTT assay and the value of optical density was measured by ELISA reader. These values were statistically analyzed by one-way ANOVA. RESULT: There was no statistical difference on MTT value between immediate and 1 day storage group. There were statistically significant differences between 1 day and 2 days tsorage, 2 and 3 days storage groups, respectively. Teeth of 3,4, and 7 days storage groups showed significantly lower MTT valuesc ompared with shorter period storage groups.
CONCLUSION
When the MTT values were substituted in standard curve, 1 day storage group at 0℃/2 MPa condition showed 68% cell viability when compared with immediate group. It dropped to 13% at 2 days, and to less than 5% at 3 days or more.

Keyword

MTT assay; Period; Periodontal ligament cell; Tooth storage

MeSH Terms

Animals
Cell Survival
Enzyme-Linked Immunosorbent Assay
Methods*
Molar
Periodontal Ligament*
Rats*
Tooth