Biomol Ther.  2015 Jan;23(1):60-70. 10.4062/biomolther.2014.086.

Effect of Cordycepin-Enriched WIB801C from Cordyceps militaris Suppressing Fibrinogen Binding to Glycoprotein IIb/IIIa

• Lee DH
• Kim HH
• Lim DH
• Kim JL
• Park HJ

• KMID: 2380364
• DOI: http://doi.org/10.4062/biomolther.2014.086

Abstract

In this study, we investigated the effects of cordycepin-enriched (CE)-WIB801C, a n-butanol extract of Cordyceps militaris-hypha on collagen-stimulated platelet aggregation. CE-WIB801C dose dependently inhibited collagen-induced platelet aggregation, and had a synergistic effect together with cordycepin (W-cordycepin) from CE-WIB801C on the inhibition of collagen-induced platelet aggregation. CE-WIB801C and cordycepin stimulated the phosphorylation of VASP (Ser157) and the dephosphorylation of PI3K and Akt, and inhibited the binding of fibrinogen to glycoprotein IIb/IIIa (alphaIIb/beta3) and the release of ATP and serotonin in collagen-induced platelet aggregation. A-kinase inhibitor Rp-8-Br-cAMPS reduced CE-WIB801C-, and cordycepin-increased VASP (Ser157) phosphorylation, and increased CE-WIB801C-, and cordycepin-inhibited the fibrinogen binding to alphaIIb/beta3. Therefore, we demonstrate that CE-WIB801C-, and cordycepin-inhibited fibrinogen binding to alphaIIb/beta3 are due to stimulation of cAMP-dependent phosphorylation of VASP (Ser157), and inhibition of PI3K/Akt phosphorylation. These results strongly indicate that CE-WIB801C and cordycepin may have preventive or therapeutic potential for platelet aggregation-mediated diseases, such as thrombosis, myocardial infarction, atherosclerosis, and ischemic cerebrovascular disease.

Keyword

CE-WIB801C; Cordycepin; VASP (Ser157); PI3K/Akt; Fibrinogen binding

MeSH Terms

1-Butanol
Adenosine Triphosphate
Atherosclerosis
Blood Platelets
Cordyceps*
Fibrinogen*
Glycoproteins*
Myocardial Infarction
Phosphorylation
Platelet Aggregation
Serotonin
Thrombosis
1-Butanol
Adenosine Triphosphate
Fibrinogen
Glycoproteins
Serotonin