Yeungnam Univ J Med.  1992 Jun;9(1):75-89. 10.12701/yujm.1992.9.1.75.

Multidrug resistance and cytotoxicity of anticancer drug by verapamil in cisplatin resistant human stomach cancer cell

• Son SK
• Kim JH

• KMID: 2377606
• DOI: http://doi.org/10.12701/yujm.1992.9.1.75

Abstract

The development of multidrug-resistant tumor cell population is a major problem in the chemotherapy of human cancer. These cells are often cross resistant to unrelated drugs and the precise mechanisms of multidrug resistant phenotype of tumor cells has not been fully elucidated. Cisplatin resistant tumor cell (SNU-1/Cisâ‚…) was induced from human stomach cancer cell line (SNU-1) in vitro. Growth profiles of survival cells were observed during 5 days by thiazolyl blue (MTT) assay. To investigate the cross resistance of various anticancer drugs in SNU-1 and SNU-1/Cis5, We compared the value of ICâ‚…â‚€-drug concentration at 50% survival of control and gained relative resistances (RR). The RR for SNC-1/Cisâ‚… were as follows; vinblastine, > 43.0; epirubicin, 22.9; dactinomycin, 16.0; etoposide, 15.0; vincristine, 9.2; adriamycin, 5.7; aclarubicin, 5.3. But 5-fluorouracil, methotrexate, daunorubicin have not cross resistance with cisplatin. Resistant inhibition values of 10µM verapamil for SNU-1/Cisâ‚… were as follows; vincristine, 13.1; epirubicin, 10.0; etoposide, 6.3; vinblastine, 4.4; dactinomycin, 3.6; daunorubicin, 2.4. Membrane proteins of 51,400 and 81,300 daltons were identified by radioiodination with SDS-PAGE, which might represented the drug resistance.

Keyword

Multidrug resistance (MDR); Verapamil; P-glycoprotein

MeSH Terms

Aclarubicin
Cell Line
Cisplatin*
Dactinomycin
Daunorubicin
Doxorubicin
Drug Resistance
Drug Resistance, Multiple*
Drug Therapy
Electrophoresis, Polyacrylamide Gel
Epirubicin
Etoposide
Fluorouracil
Humans*
In Vitro Techniques
Membrane Proteins
Methotrexate
P-Glycoprotein
Phenotype
Stomach Neoplasms*
Stomach*
Verapamil*
Vinblastine
Vincristine
Aclarubicin
Cisplatin
Dactinomycin
Daunorubicin
Doxorubicin
Epirubicin
Etoposide
Fluorouracil
Membrane Proteins
Methotrexate
P-Glycoprotein
Verapamil
Vinblastine
Vincristine