Yeungnam Univ J Med.  1991 Dec;8(2):138-149. 10.12701/yujm.1991.8.2.138.

Existance of cholinergic and purinergic receptor on the detrusor muscle of rat urinary bladder

• Choi TS
• Kwon OC
• Ha JH
• Lee KY
• Kim WJ

• KMID: 2377369
• DOI: http://doi.org/10.12701/yujm.1991.8.2.138

Abstract

This study was aimed at investigation of the stimulatory innervations on the rat urinary bladder. Detrusor muscle strips of 15 mm long were suspended in isolated muscle chambers containing 1 ml of PSS maintained at 37℃ and aerated with 95% O²/5% Co². Isometric myography was performed, and the results were as followings: Muscle strips showed "on-contraction" by electric field stimulation (EFS) frequency-dependently. The EFS-induced contraction was not affected by hexamethonium, a ganglion blocker, but abolished by tetrodotoxin, a nerve conduction blocker. Physostigmine, a cholinesterase inhibitor enhanced the EFS-induced contraction which was inhibited by hemicholinium, an inhibitor of choline uptake at the cholinergic nerve ending. Such an EFS-induced contraction was antagonized by atropine only partially, and the atropine-resistant portion was completely abolished by the desensitization of purinergic receptors by prolonged incubating of the strips in the presence of high concentration of ATP. Bethanechol, a cholinergic agonist, elicited concentration-dependent contraction. Adenosine triphosphate (ATP), a purinergic agonist, induced a weak but concentration-dependent contraction of short duration. Bethanechol-induced contraction was not affected by ATP-desensitization, and ATP-induced contraction was not affected by tetrodotoxin. These results suggest that there are at least two main stimulatory components of innervations in the detrusor muscle, cholinergic muscarinic and purinergic; and those receptors are independent each other.

Keyword

Stimulatory component; Innervation; Detrusor muscle; Cholinergic; Purinergic; Independent

MeSH Terms

Adenosine Triphosphate
Animals
Atropine
Bethanechol
Choline
Cholinergic Agonists
Cholinesterases
Ganglion Cysts
Hemicholinium 3
Hexamethonium
Myography
Nerve Endings
Neural Conduction
Physostigmine
Rats*
Receptors, Purinergic
Tetrodotoxin
Urinary Bladder*
Adenosine Triphosphate
Atropine
Bethanechol
Choline
Cholinergic Agonists
Cholinesterases
Hemicholinium 3
Hexamethonium
Physostigmine
Receptors, Purinergic
Tetrodotoxin