Korean J Urol.
2005 May;46(5):509-517.
Bladder Dysfunction in Focal Cerebral Ischemia of Rat
- Affiliations
-
- 1Department of Urology, Seoul National University Boramae Hospital, Seoul, Korea. hchoi@snu.ac.kr
- 2Department of Neurosurgery, Seoul National University Boramae Hospital, Seoul, Korea.
- 3Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
- 4Department of Neurology, Seoul National University School of Medicine, Seoul, Korea.
- 5Department of Urology, Seoul National University School of Medicine, Seoul, Korea.
Abstract
- Purpose
The purpose of this study was to evaluate the active contractile properties of detrusor smooth muscle from bladders of cerebral-infarcted rats (CI rats).
Materials and Methods
The cerebral infarction was induced in 8 to 9 week-old male Sprague-Dawley rats by occlusion of the left middle cerebral artery. At 2 and 4 weeks after the operation, bladder muscle strips were evaluated for force development in response to electrical field, 5 micrometer carbachol (Cch) and 60mM KCl stimulation using an isometric transducer. The contractile response to field stimulation on the additions of 1micrometer atropine and 5micrometer alpha, beta-methylene ATP (abmATP) were measured, and the contributions of cholinergic and purinergic transmissions determined. Cystometrography (CMG) was also performed in anaesthetized rats.
Results
In comparison with sham-operated rats, the CI rats showed a shorter voiding interval, smaller peak voiding pressure, smaller voiding volume and greater residual urine volume on CMG. The CI rats also showed smaller tension at maximum contraction induced by Cch and a quicker response to maximum contraction induced by KCl than the sham operated rats. The CI rats showed time-dependent increases and decreases in the proportions of abmATP-sensitive (purinergic) and atropine-sensitive (cholinergic) components, respectively.
Conclusions
CI rats showed changes in detrusor activity, contractility and the proportions of cholinergic and purinergic components. The increase in the purinergic contraction of the detrusor muscle was believed to be related to bladder instability. The results of this study will be useful in explaining detrusor hyperactivity, with impaired contractility (DHIC), in patients with cerebral infarction.
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