Korean J Endocr Surg.  2012 Jun;12(2):87-91. 10.0000/kjes.2012.12.2.87.

Detection of BRAF(V600E) Mutation using Peptide Nucleic Acid-Mediated PCR Clamping Method in Fine Needle Aspiration Cytology

• Hong JC
• Park HS
• Kang MK
• Lee JH
• Kim DK
• Park MK
• Kang DY
• Kim SH
• Hong SH
• Kim DC

• KMID: 2375355
• DOI: http://doi.org/10.0000/kjes.2012.12.2.87

Abstract

PURPOSE
The aim of this study was to evaluate the diagnostic value of a peptide nucleic acid (PNA)-mediated PCR clamping method for the detection of BRAFV600E mutations in fine needle aspiration cytology (FNAC).
METHODS
One hundred sixty four patients underwent FNAC to evaluate BRAFV600E mutations between April 2011 and November 2011. Among them, forty-two patients were diagnosed with papillary thyroid carcinoma in a permanent pathologic specimen. A PNA-mediated PCR clamping method and a Dual-Priming Oligonucleotide (DPO)-based Real-time PCR method were used to detect the BRAFV600E mutation. We compared the result of mutation between the two methods.
RESULTS
A BRAF mutation was found in 31 samples created by the PNA-mediated PCR clamping method, and in 28 samples in the DPO-based Real-time PCR method. The rate of BRAF mutation was 73.8% in association with the PNA-mediated PCR clamping method, and 66.7% in association with the DPO-based Real-time PCR method. There was no statistical differences between the two methods (P>0.05).
CONCLUSION
The PNA-mediated PCR clamping method may be an alternative to the DPO-based Real-Time PCR method for detection of BRAF mutations in thyroid nodules.

Keyword

Thyroid cancer; BRAF mutation

MeSH Terms

Biopsy, Fine-Needle*
Constriction*
Humans
Methods*
Polymerase Chain Reaction*
Real-Time Polymerase Chain Reaction
Thyroid Neoplasms
Thyroid Nodule