Yonsei Med J.  2016 Mar;57(2):469-474. 10.3349/ymj.2016.57.2.469.

Expression of CAIII and Hsp70 Is Increased the Mucous Membrane of the Posterior Commissure in Laryngopharyngeal Reflux Disease

• Min HJ
• Hong SC
• Yang HS
• Mun SK
• Lee SY

• KMID: 2374055
• DOI: http://doi.org/10.3349/ymj.2016.57.2.469

Abstract

PURPOSE
We tried to evaluate the difference in the expression of carbonic anhydrase (CA) III and heat shock protein (Hsp) 70 between laryngopharyngeal reflux disease (LPRD) and non-LPRD patients.
MATERIALS AND METHODS
The study involved 28 patients who underwent laryngeal microsurgery due to benign laryngeal disease from March to August 2008. Reflux symptom index (RSI) and reflux finding score (RFS) were measured for each person, and they were assigned either to the LPRD group (n=10) or non-LPRD group (n=18). Tissue samples were obtained from the mucosa of posterior commissure, and immunohistochemistry (IHC) staining of CAIII and Hsp70 was performed. The IHC scores were measured and compared with clinical features including RSI and RFS.
RESULTS
Total 10 patients were assigned as LPRD group, and 18 patients were as control group. The mean IHC score of CAIII and Hsp70 was 1.70+/-1.06 and 1.90+/-0.88, respectively, in LPRD patients, whereas the mean IHC score of CAIII and Hsp70 was 0.78+/-0.73 and 0.94+/-0.87, respectively, in non-LPRD patients. The difference between two groups was statistically significant (p<0.05).
CONCLUSION
CAIII and Hsp70 expressions were higher in LPRD patients that in non-LPRD patients, suggesting the possibility as one of biomomarker in LPRD diagnosis.

Keyword

Carbonic anhydrase-III; heat shock protein 70; laryngopharyngeal reflux disease; deglutition; deglutition disorders

MeSH Terms

Adult
Aged
Biopsy
Carbonic Anhydrase III/*metabolism
Case-Control Studies
Female
HSP70 Heat-Shock Proteins/*metabolism
Humans
Immunohistochemistry
Laryngopharyngeal Reflux/*diagnosis/surgery
Laryngoscopes
Laryngoscopy
Larynx
Male
Middle Aged
Mucous Membrane/*metabolism
Carbonic Anhydrase III
HSP70 Heat-Shock Proteins

Figure

• 

  Fig. 1 Immunohistochemical (IHC) staining for CAIII and Hsp70 in the posterior commissure epithelium (×200). IHC reactivity for CAIII and Hsp70 was read by our scoring system, based on the quantity of brown pigmentation in the cytoplasm of cells. (A) Not expressed in all cells, showing IHC score 0. (B) Present less than 50% cells, showing IHC score 1. (C) Present between 50% and 90% cells, showing IHC score 2. (D) Expressed more than 90% of posterior commissure epithelial cells, showing IHC score 3. CAIII, carbonic anhydrase-III; Hsp70, heat shock protein 70.

• 

  Fig. 2 IHC score of CAIII in LPRD and non-LPRD patients. In LPRD group (n=10), the semiquantitative IHC staining score of CAIII was 0 (n=2), 1 (n=1), 2 (n=5), and 3 (n=2). In non-LPRD group (n=12), IHC staining score of CAIII was 0 (n=1), 1 (n=8), and 2 (n=3). IHC, immunohistochemical; CAIII, carbonic anhydrase-III; LPRD, laryngopharyngeal reflux disease.

• 

  Fig. 3 IHC score of Hsp70 in LPRD and non-LPRD patients. The IHC staining scores of Hsp70 for the LPRD patients were 0 (n=1), 1 (n=1), 2 (n=6), and 3 (n=2). The IHC staining scores of Hsp70 for the 18 non-LPRD patients were 0 (n=6), 1 (n=8), 2 (n=3), and 3 (n=1). IHC, immunohistochemical; Hsp70, heat shock protein 70; LPRD, laryngopharyngeal reflux disease.

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