Ann Lab Med.  2016 Sep;36(5):499-500. 10.3343/alm.2016.36.5.499.

Basophil Activation Test for Chronic Urticaria

Affiliations
  • 1Department of Medicine-Unit of Geriatry, University of Verona, Verona, Italy. salvatore.chirumbolo@univr.it

Abstract

No abstract available.


MeSH Terms

Basophils/cytology/*metabolism
Flow Cytometry
HLA-DR Antigens/metabolism
Humans
Interleukin-3 Receptor alpha Subunit/metabolism
Leukocyte Count
Phosphoric Diester Hydrolases/metabolism
Pyrophosphatases/metabolism
Receptors, CCR3/metabolism
Urticaria/*diagnosis/immunology/metabolism
HLA-DR Antigens
Interleukin-3 Receptor alpha Subunit
Phosphoric Diester Hydrolases
Pyrophosphatases
Receptors, CCR3

Figure

  • Fig. 1 Flow cytometry showing dot plots of basophils collected from two representative healthy blood donors. Basophils were gated according to reference 7. Panels A, E: T and B lymphocytes; B, F: plasmacytoid dendritic cells; C, G: monocytes; D, H: basophils. Number of gated CD123bright/HLADRneg basophils: 504 and 523, respectively. For further information, see references 2 and 7.


Cited by  1 articles

Reply to the Letter to the Editor by Dr. Chirumbolo
Dong Il Won
Ann Lab Med. 2016;36(5):498-498.    doi: 10.3343/alm.2016.36.5.498.


Reference

1. Kim Z, Choi BS, Kim JK, Won DI. Basophil markers for identification and activation in the indirect basophil activation test by flow cytometry for diagnosis of autoimmune urticaria. Ann Lab Med. 2016; 36:28–35. PMID: 26522756.
2. Chirumbolo S, Ortolani R, Vella A. CCR3 as a single selection marker compared to CD123/HLADR to isolate basophils in flow cytometry: some comments. Cytometry A. 2011; 79:102–106. PMID: 21265004.
3. Monneret G. CCR3 for basophil activation test: a necessary but insufficient step. Clin Exp Allergy. 2010; 40:953. author reply 954. PMID: 20557552.
4. Colonna M, Trinchieri G, Liu YJ. Plasmacytoid dendritic cells in immunity. Nat Immunol. 2004; 5:1219–1226. PMID: 15549123.
5. Beaulieu S, Robbiani DF, Du X, Rodrigues E, Ignatius R, Wei Y, et al. Expression of a functional eotaxin (CC chemokine ligand 11) receptor CCR3 by human dendritic cells. J Immunol. 2002; 169:2925–2936. PMID: 12218106.
6. Chirumbolo S. Basophil activation test in allergy: time for an update? Int Arch Allergy Immunol. 2012; 158:99–114. PMID: 22269476.
7. Chirumbolo S, Vella A, Ortolani R, De Gironcoli M, Solero P, Tridente G, et al. Differential response of human basophil activation markers: a multi-parameter flow cytometry approach. Clin Mol Allergy. 2008; 6:12. PMID: 18925959.
8. Chirumbolo S. The use of IL-3 in basophil activation tests is the real pitfall. Cytometry B ClinCytom. 2011; 80:137–138. author reply 139.
9. Chirumbolo S. CD203c in patients with cystic fibrosis and allergy to Aspergillus fumigatus. J Allergy Clin Immunol. 2016; 137:969. PMID: 26782973.
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