Int J Oral Biol.  2016 Dec;41(4):199-208. 10.11620/IJOB.2016.41.4.199.

Identification of Non-Aggregatibacter actinomycetemcomitans Bacteria Grown on the Tryptic soy-Serum-Bacitracin-Vancomycin Medium

• Jo E
• Park SN
• Kook JK

• KMID: 2368583
• DOI: http://doi.org/10.11620/IJOB.2016.41.4.199

Abstract

The aim of this study was to identify the non-Aggregatibacter actinomycetemcomitans bacteria grown on the tryptic soy-serum-bacitracin-vancomycin (TSBV) medium, an A. actinomycetemcomitans selective medium. A total of 82 unidentified bacterial isolates from the oral cavities of a Korean population were kindly provide by the Korean Collection for Oral Microbiology. All the clinical isolates were grown on TSBV medium and bacterial DNA purified from each isolate was subjected to PCR with universal primers specific for bacterial 16S rRNA genes (16S rDNAs) sequence. The each bacterial 16S rDNA was amplified by PCR and the nucleotide sequences of it was determined by the dideoxynucleotide chain termination method. They were identified by 16S rDNA sequence comparison method at the specie-level. The data showed that Neisseria spp. (42 strains), Fusobacterium spp. (10 strains), Capnocytophaga spp. (8 strains), Propionibacterium acnes (5 strains), Aggregatibacter aprophilus (4 strains), Campylobacter spp. (5 strains), Veillonella dispar (3 strains), Streptococcus sp. (1 strain), Haemophilus parainfluenzae (1 strain), Leptotrichia wadei (1 strain), Morococcus sp./Neisseria sp. (1 strain), and Staphylococcus sp. (1 strain) were identified. These results could be used to develop a new A. actinomycetemcomitans-selective medium which is more effective than the TSBV medium in future studies.

Keyword

tryptic soy-serum-bacitracin-vancomycin medium; non-Aggregatibacter actinomycetemcomitans; identification; 16S rDNA

MeSH Terms

Aggregatibacter
Bacteria*
Base Sequence
Campylobacter
Capnocytophaga
DNA, Bacterial
DNA, Ribosomal
Fusobacterium
Genes, rRNA
Haemophilus parainfluenzae
Leptotrichia
Methods
Neisseria
Polymerase Chain Reaction
Propionibacterium acnes
Staphylococcus
Streptococcus
Veillonella
DNA, Bacterial
DNA, Ribosomal