Yonsei Med J.  2015 Jul;56(4):1106-1113. 10.3349/ymj.2015.56.4.1106.

Fracture Healing Effects of Locally-Administered Adipose Tissue-Derived Cells

Affiliations
  • 1Department of Radiology, Gil Hospital, Gachon University School of Medicine and Science, Incheon, Korea.
  • 2Department of Nuclear Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea. tjeonnm@yuhs.ac
  • 3Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA.

Abstract

PURPOSE
Although the applications of adipose tissue-derived cells (ADCs) in regenerative medicine have been investigated, the role of ADCs in fracture healing remains unclear. In this study, we examined the fracture-healing effects and survival of transplanted ADCs using micro-computed tomography (CT) and bioluminescence imaging (BLI).
MATERIALS AND METHODS
Luciferase-expressing ADCs were suspended in solubilized basement membrane preparation (SBMP) and xenografted on defects in the right femur of nude mice (n=5). SBMP alone was grafted on a defect in the contralateral femur. Serial in vivo micro-CT and BLI were performed for 20 days. Ex vivo BLI images of both femurs were obtained. Differences in the Hounsfield unit (HU), HUratio, and luciferase activities were compared using Wilcoxon signed-rank tests and non-parametric longitudinal analyses (p<0.05).
RESULTS
In vivo BLI revealed a signal drop on day 2, reconstitution on day 5, and continuous decrement thereafter. Ex vivo BLI revealed residual activity in the ADC-implanted and adjacent areas. No activity was detected in the contralateral femur. The overall increment rate of normalized HUs was higher for ADC-treated femurs than for SBMP-treated femurs. Cell migration to distant injury sites was not detected.
CONCLUSION
Enhanced bone density in the implant area suggests that ADCs have fracture-healing effects.

Keyword

Fracture; stem cells; computed tomography; imaging

MeSH Terms

Adipose Tissue/*transplantation
Animals
Cell Movement
Cells, Cultured
Femur
*Fracture Healing
Mice
Mice, Nude
Tomography, X-Ray Computed
X-Ray Microtomography

Figure

  • Fig. 1 Bioluminescence imaging of adipose tissue-derived cell-implanted fracture model. A region of interest (ROI) that included nearly the entire signal was drawn on the image recorded in the left lateral decubitus position.

  • Fig. 2 Micro-computed tomography (CT) of bilateral femurs with holes (diameter, 0.5 mm). Baseline micro-CT images of control (A) and adipose tissue-derived cell (ADC)-implanted sides (B) showed relatively low bone densities in the holes. Final micro-CT images on day 20 showed increased bone densities in both the control (C) and ADC-implanted sides (D).

  • Fig. 3 The time vs. activity curve for firefly luciferase-expressing ADCs showed rapid decrement on post-operative day (POD) 7, followed by slow decrement until POD 12, before reaching a plateau. BLI, bioluminescence imaging; ADCs, adipose tissue-derived cells.

  • Fig. 4 Ex vivo BLI revealed no luciferase activity in the left femur. However, in three out of five mice, luciferase activity was detected in the primary site on the right femur as well as in adjacent areas (A, C, and D), while in the remaining two, activity was detected only in the primary sites (B and E). BLI, bioluminescence imaging.

  • Fig. 5 (A) There was no significant difference in the Hounsfield units (HUs) of the right and left femurs until day 20. (B) However, there was a significant difference in the HUratio (normalized to the baseline values) of the right and left femurs on day 20. POD, post-operative day; ADC, adipose tissue-deriviced cell.


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Mohammad Mahdi Vashghani Farahani, Reza Ahadi, Mohammadamin Abdollahifar, Mohammad Bayat
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