Allergy.  1995 Sep;15(3):320-329.

Effect of eicosapentanoic acid on neutrophil-endothelial adhesion

• Kwon YD
• Hwang JB
• Han CH
• Chung HL
• Lee SJ
• An YH

Abstract

Adhesion molecule may play a primary role in the pathogenesis of bronchial asthma. ELAM - 1 (endothelial leukocyte adhesion molecule- 1) which is a member of selectin family has known to be induced by cytokine or endotoxin and diate neutrophil migration and adhesion to vascular endothelium. Recent studies demonstrate ELAM-1 is induced exclusively on bronchial mucosal vascular endothelium 6 hours after single allergen inhalation which correlates with the influx of neutrophils into lung and onset of late phase airway obstruction. The major N-3 fatty acid constituent of fish oil, eicosapentaenoic acid(EPA) has known to have antiinflammatory effect by inhibiting lipoxygenase pathway of arachidonic acid and dietary supplementation with fish oil lipid for 10 weeks significantly attenuated allergen induced late asthmatic response without a significant change in clinical course. EPA was hypothesized to downregulate the expression of celt adhesion molecule and reduce inflammatory reaction of late allergic response. Human neutrophils were isolated from peripheral blood by percoll gradient centrifuge method and suspended in RPMI medium to do adhesion assay. Confluent human umbilical vein endothelial cells(HUVECs) in 96 well plate were stimulated with lipopolysaccaride(LPS 50 ug/ml) for 5 hours to induce adhesion molecules. When oxidized EPA was added to the medium of HUV-ECs 1 hour before LPS-stimulation, adhesion of neutrophils to HUVECs was markedly decreased and the expression of ELAM-1 m-RNA was also reduced to the level of unstimulated HUVECs. In conclusion, exposure to oxidized EPA inhibited the adhesion of neutrophils to vascular endothelial cells and oxidized EPA was thought to modulate allergic inflammatory response by reducing ELAM-1 expression which is related to the influx of neutrophils at the onset of late allergic response.

MeSH Terms

Airway Obstruction
Arachidonic Acid
Asthma
Dietary Supplements
E-Selectin
Eicosapentaenoic Acid*
Endothelial Cells
Endothelium, Vascular
Humans
Inhalation
Leukocytes
Lipoxygenase
Lung
Neutrophils
Umbilical Veins
Arachidonic Acid
E-Selectin
Eicosapentaenoic Acid
Lipoxygenase